Difference between revisions of "DNA Melting: DNA Sequences"
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| 20bp-single mismatch || AGT TTC GAT TGC TGC TTG AT || 6129 || 40 | | 20bp-single mismatch || AGT TTC GAT TGC TGC TTG AT || 6129 || 40 | ||
|- | |- | ||
| − | | 20bp-double mismatch || AGT TTC GAT TGA TGC TTG AT || | + | | 20bp-double mismatch || AGT TTC GAT TGA TGC TTG AT || 6153 || 35 |
|- | |- | ||
| 20bp-complete mismatch || CAC CGA CGT ACT ATT CCT AA || 6021 || 45 | | 20bp-complete mismatch || CAC CGA CGT ACT ATT CCT AA || 6021 || 45 | ||
Revision as of 18:31, 9 October 2013
Single-stranded DNA sequences:
| Name | Sequence | MW | GC % |
|---|---|---|---|
| 20bp | ATC AAG CAG CCA TCG AAA CT | 6079 | 45 |
| 20bp-comp | AGT TTC GAT GGC TGC TTG AT | 6154 | 45 |
| 20bp-single mismatch | AGT TTC GAT TGC TGC TTG AT | 6129 | 40 |
| 20bp-double mismatch | AGT TTC GAT TGA TGC TTG AT | 6153 | 35 |
| 20bp-complete mismatch | CAC CGA CGT ACT ATT CCT AA | 6021 | 45 |
| 30bp | ATC AAG CAG CCA TCG AAA CTT AGG CTT ACA | 9168 | 43.3 |
| 30bp-comp | TGT AAG CCT AAG TTT CGA TGG CTG CTT GAT | 9243 | 43.3 |
| 40bp | ATC AAG CAG CCA TCG AAA CTT AGG CTT ACA ACC AGT GAC T | 12242 | 45 |
| 40bp-comp | AGT CAC TGG TTG TAA GCC TAA GTT TCG ATG GCT GCT TGA T | 12348 | 45 |
For double-stranded DNA solutions, one of the 20bp, 30bp, or 40bp sequences is mixed in equal concentration with its respective complement, e.g., 30bp and 30bp-comp, in a desired saline solution. For 20bp mismatch solutions, the 20bp sequence is mixed in equal concentration with one of the mismatch sequences.
