Difference between revisions of "Assignment 6 Overview"

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During the next lab exercise on measuring DNA melting curves, you will build and debug several electronic circuits. This assignment will introduce you to the electronic components and test equipment you will use.  
 
During the next lab exercise on measuring DNA melting curves, you will build and debug several electronic circuits. This assignment will introduce you to the electronic components and test equipment you will use.  
This bootcamp requires an understanding of basic circuits. If you need to review circuit concepts, start with the [[Electronics Primer]] page. If you have a lot of experience with electronics, ask one of the instructors about doing a stimulating mini-project instead of the mini-lab assignment.
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This assignment requires an understanding of basic circuits. If you need to review circuit concepts, start with the [[Electronics Primer]] page. If you have a lot of experience with electronics, ask one of the instructors about doing a stimulating mini-project instead of this assignment.
 
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A short answer-book style writeup is required. Your writeup should include the practice problems and any '''bolded questions''' asked throughout the lab instructions. Don't forget the basics: report measurements with an appropriate number of significant figures, units, and uncertainty. Label plot axes.
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== Assignment Details==
 
== Assignment Details==
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# Part 2: [[Assignment 6, Part 2: Electronics bootcamp|Get in the lab and start building and measuring basic circuits]];
 
# Part 2: [[Assignment 6, Part 2: Electronics bootcamp|Get in the lab and start building and measuring basic circuits]];
 
# Part 3: [[Assignment 6, Part 3: Transimpedance amplifier|Build an amplifier circuit]] that you'll use to measure the amount of fluorescence from your DNA melting samples.
 
# Part 3: [[Assignment 6, Part 3: Transimpedance amplifier|Build an amplifier circuit]] that you'll use to measure the amount of fluorescence from your DNA melting samples.
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{{Template:Assignment Turn In|message= all of your work (comprehensive list below) on Stellar in a single PDF file named <lastname><firstname>Assignment6.pdf.}}
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Turn in:
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# answers to the [[Assignment 6, Part 1: Pre-lab questions|prelab questions]]
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# answers to the following voltage divider questions:
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#* Record the values you selected for R<sub>1</sub> and R<sub>2</sub>.
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#* Find the gain of the circuit, <math>^{V_{out}}/_{V_{in}}</math>
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#* Plot an ''I-V'' curve with ''I'' on the vertical axis and ''V<sub>in</sub>'' on the horizontal axis, over the range 0 V <  ''V<sub>in</sub>'' < 15 V. (A hand-drawn plot is fine.)
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#* What is the maximum power dissipated in each resistor between 0 V <  ''V<sub>in</sub>'' < 15 V?
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# the measured values of your resistors.
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# your measured voltages ''V<sub>out</sub>'' for ''V<sub>in</sub>'' = 0, 2.5, 5, 10 and 15 V.
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# your measured voltage across ''R<sub>2</sub>'' in the presence of the 1k&Omega resistor. By what percentage did the measurement change?
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# Recorded values of the current through the circuit for ''V<sub>in</sub>'' = 0, 2.5, 5, 10 and 15 V
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# A plot of the measured ''I-V'' curve on the same set of axes as the calculated curve.
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# your choice of capacitor and resistor values and corresponding Bode plot. Make sure to label the cutoff frequency.
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# a plot of the measured values of <math>\frac{V_{out}}{V_{in}}</math> versus frequency on the same set of axes as your Bode plot.
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# your Bode gain and phase plots and the corresponding circuit diagram for each of the four circuits A through D. Optional: In Matlab, use <tt>nlinfit</tt> to fit parameters of a transfer function for each circuit.
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==Background Reading==
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*[[Electronics Primer]]

Revision as of 19:06, 29 August 2017

20.309: Biological Instrumentation and Measurement

ImageBar 774.jpg


Photo by Brendan Dolan-Gavitt

During the next lab exercise on measuring DNA melting curves, you will build and debug several electronic circuits. This assignment will introduce you to the electronic components and test equipment you will use. This assignment requires an understanding of basic circuits. If you need to review circuit concepts, start with the Electronics Primer page. If you have a lot of experience with electronics, ask one of the instructors about doing a stimulating mini-project instead of this assignment.

Assignment Details

This assignment has three parts:

  1. Part 1: A few questions to answer before you start your lab work.;
  2. Part 2: Get in the lab and start building and measuring basic circuits;
  3. Part 3: Build an amplifier circuit that you'll use to measure the amount of fluorescence from your DNA melting samples.


Pencil.png

all of your work (comprehensive list below) on Stellar in a single PDF file named <lastname><firstname>Assignment6.pdf.


Turn in:

  1. answers to the prelab questions
  2. answers to the following voltage divider questions:
    • Record the values you selected for R1 and R2.
    • Find the gain of the circuit, $ ^{V_{out}}/_{V_{in}} $
    • Plot an I-V curve with I on the vertical axis and Vin on the horizontal axis, over the range 0 V < Vin < 15 V. (A hand-drawn plot is fine.)
    • What is the maximum power dissipated in each resistor between 0 V < Vin < 15 V?
  3. the measured values of your resistors.
  4. your measured voltages Vout for Vin = 0, 2.5, 5, 10 and 15 V.
  5. your measured voltage across R2 in the presence of the 1k&Omega resistor. By what percentage did the measurement change?
  6. Recorded values of the current through the circuit for Vin = 0, 2.5, 5, 10 and 15 V
  7. A plot of the measured I-V curve on the same set of axes as the calculated curve.
  8. your choice of capacitor and resistor values and corresponding Bode plot. Make sure to label the cutoff frequency.
  9. a plot of the measured values of $ \frac{V_{out}}{V_{in}} $ versus frequency on the same set of axes as your Bode plot.
  10. your Bode gain and phase plots and the corresponding circuit diagram for each of the four circuits A through D. Optional: In Matlab, use nlinfit to fit parameters of a transfer function for each circuit.

Background Reading