Difference between revisions of "20.109(S24):Class data"

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(→‎Module 1: Drug discovery)
(→‎Module 1: Drug discovery)
Line 10: Line 10:
  
 
Please find the SMM screen results [[Media:Screen results.csv | here]].
 
Please find the SMM screen results [[Media:Screen results.csv | here]].
 +
  
 
'''DSF results:'''
 
'''DSF results:'''
Line 21: Line 22:
 
| Replicate #3
 
| Replicate #3
 
| Average T<sub>m</sub>
 
| Average T<sub>m</sub>
 +
|-
 +
| '''TR Orange'''
 +
|-
 +
| DMSO
 +
|
 +
|
 +
|
 +
|
 +
|-
 +
| Small molecule #1
 +
|
 +
|
 +
|
 +
|
 +
|-
 +
| Small molecule #2
 +
|
 +
|
 +
|
 +
|
 +
|-
 +
| Small molecule #3
 +
|
 +
|
 +
|
 +
|
 +
|-
 +
| Small molecule #4
 +
|
 +
|
 +
|
 +
|
 
|-
 
|-
 
| '''TR Yellow'''
 
| '''TR Yellow'''
 
|-
 
|-
 
| DMSO
 
| DMSO
−
| 44.02929134
+
|  
−
| 45.55669291
+
|  
−
| 45.04755906
+
|  
−
| 45.55669291
+
|  
 
|-
 
|-
 
| Small molecule #1
 
| Small molecule #1
−
| 46.06582677
+
|  
−
| 45.55669291
+
|  
−
| 45.04755906
+
|  
−
| 43.52015748
+
|  
 +
|-
 +
| Small molecule #2
 +
|
 +
|
 +
|
 +
|
 +
|-
 +
| Small molecule #3
 +
|
 +
|
 +
|
 +
|
 +
|-
 +
| Small molecule #4
 +
|
 +
|
 +
|
 +
|
 
|-
 
|-
 
| '''TR Green'''
 
| '''TR Green'''
 
|-
 
|-
−
| FK296
+
| DMSO
−
| 44.01818898
+
|  
−
| 45.54488189
+
|  
−
| 45.54488189
+
|  
−
| 45.54488189
+
|  
−
| 46.567717
+
−
| 46.05377953
+
−
| 45.54488189
+
−
| 46.05377953
+
−
| 46.56267717
+
 
|-
 
|-
−
| FK502
+
| Small molecule #1
−
| 46.05
+
|  
−
| 46.05
+
|  
−
| 43.51
+
|  
−
| 43.51
+
|  
−
| 43.00
+
|-
−
| 43.00
+
| Small molecule #2
−
| 43.51
+
|
−
| 43.51
+
|
−
| 43.51
+
|
 +
|
 +
|-
 +
| Small molecule #3
 +
|
 +
|
 +
|
 +
|
 +
|-
 +
| Small molecule #4
 +
|  
 +
|  
 +
|  
 +
|  
 
|-
 
|-
 
| '''TR Blue'''
 
| '''TR Blue'''
 
|-
 
|-
−
| FK141
+
| DMSO
−
| 38.93
+
|  
−
| 44.538
+
|  
−
| 45.047
+
|  
−
| 46.065
+
|  
−
| 46.575
+
−
| 47.593
+
−
| 46.574
+
−
| 46.574
+
−
| 46.066
+
 
|-
 
|-
−
| FK189
+
| Small molecule #1
−
| 46.574
+
|  
−
| 47.084
+
|  
−
| 46.574
+
|  
−
| 46.574
+
|  
−
| 46.574
+
−
| 46.066
+
−
| 45.557
+
−
| 42.502
+
−
| 42.501
+
 
|-
 
|-
−
| '''TR Purple'''
+
| Small molecule #2
 +
|
 +
|
 +
|
 +
|
 
|-
 
|-
−
| FK189
+
| Small molecule #3
−
| 45.05
+
|  
−
| 44.54
+
|  
−
| 45.05
+
|  
−
| 44.54
+
|  
−
| 44.54
+
−
| 44.03
+
−
| 40.47
+
−
| 43.01
+
−
| 45.05
+
 
|-
 
|-
−
| FK502
+
| Small molecule #4
−
| 45.56
+
|  
−
| 44.54
+
|  
−
| 45.56
+
|  
−
| 45.05
+
|  
−
| 46.57
+
−
| 43.52
+
−
| 44.54
+
−
| 46.07
+
−
| 45.56
+
 
|-
 
|-
−
| '''WF Green'''
+
| '''TR Pink'''
 
|-
 
|-
−
| FK189
+
| DMSO
−
| 47.580
+
|  
−
| 47.580
+
|  
−
| 47.072
+
|  
−
| 46.563
+
|  
−
| 46.563
+
−
| 47.072
+
−
| 48.089
+
−
| 48.089
+
−
| 47.580
+
 
|-
 
|-
−
| FK296
+
| Small molecule #1
−
| 44.018
+
|  
−
| 42.491
+
|  
−
| 47.580
+
|  
−
| 47.580
+
|  
−
| 47.072
+
−
| 47.580
+
−
| 49.616
+
−
| 49.616
+
−
| 50.634
+
 
|-
 
|-
−
| '''WF Blue'''
+
| Small molecule #2
 +
|
 +
|
 +
|
 +
|
 
|-
 
|-
−
| FK141
+
| Small molecule #3
−
| 46.09
+
|  
−
| 46.09
+
|  
−
| 46.60
+
|  
−
| 47.11
+
|  
−
| 40.00
+
−
| 47.11
+
−
| 47.11
+
−
| 47.11
+
−
| 47.11
+
 
|-
 
|-
−
| FK296
+
| Small molecule #4
−
| 45.08
+
|  
−
| 43.55
+
|  
−
| 47.11
+
|  
−
| 43.04
+
|  
−
| 47.11
+
−
| 47.11
+
−
| 57.26
+
−
| 57.26
+
−
| 42.54
+
 
|-
 
|-
 
|}
 
|}

Revision as of 19:07, 29 February 2024

20.109(S24): Laboratory Fundamentals of Biological Engineering

Sp24 banner image.png

Spring 2024 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       M1: Drug discovery        M2: Protein engineering        M3: Project design       


Module 1: Drug discovery

Please find uploaded data for Mod 1 in the Dropbox folder linked [here].


Please find the SMM screen results here.


DSF results:

Replicate #1 Replicate #2 Replicate #3 Average Tm
TR Orange
DMSO
Small molecule #1
Small molecule #2
Small molecule #3
Small molecule #4
TR Yellow
DMSO
Small molecule #1
Small molecule #2
Small molecule #3
Small molecule #4
TR Green
DMSO
Small molecule #1
Small molecule #2
Small molecule #3
Small molecule #4
TR Blue
DMSO
Small molecule #1
Small molecule #2
Small molecule #3
Small molecule #4
TR Pink
DMSO
Small molecule #1
Small molecule #2
Small molecule #3
Small molecule #4

Module 2: Protein engineering

Please find uploaded data for Mod 2 in the Dropbox folder linked here.
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