Difference between revisions of "20.109(S23):Spring 2023 schedule"

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| M2D8
 
| M2D8
 
| T/W Apr 25/26
 
| T/W Apr 25/26
| [http://be.mit.edu/directory/becky-meyer BCM] <br>
+
| Comm Lab <br>
 
| [[20.109(S23):M2D8 |Complete data analysis and organize Research article figures]] <br>  
 
| [[20.109(S23):M2D8 |Complete data analysis and organize Research article figures]] <br>  
 
| <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M2D8|Homework due]]
 
| <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M2D8|Homework due]]
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| M3D1
 
| M3D1
 
| R/F Apr 27/28
 
| R/F Apr 27/28
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>  
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| [http://be.mit.edu/directory/becky-meyer BCM]<br>  
 
| [[20.109(S23):M3D1 |Brainstorm ideas for Research proposal presentation]] <br>  
 
| [[20.109(S23):M3D1 |Brainstorm ideas for Research proposal presentation]] <br>  
 
| [[20.109(S23):Homework#Due_M3D1|Homework due]] <br>
 
| [[20.109(S23):Homework#Due_M3D1|Homework due]] <br>

Revision as of 19:15, 1 February 2023

20.109(S23): Laboratory Fundamentals of Biological Engineering

Sp23 banner image v2.png

Spring 2023 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       M1: Drug discovery        M2: Protein engineering        M3: Project design       


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not enough time to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times: Tuesday (T) and Thursday (R) 11 - 12 pm in 1-390
Laboratory section times: Tuesday (T) and Thursday (R) 1 - 5 pm or Wednesday (W) and Friday (F) 1 - 5 pm in 56-322

ABSENCE POLICY:
Absences from lecture: Attendance will be recorded for participation points throughout the semester. If absent, student is responsible for all information provided in lecture.
Absences from laboratory: Excused absences should be discussed with the Instructors as soon as possible. Because make-up laboratory time is not provided, attendance in another section may be required to complete the necessary experiments. Unexcused absences will result in a 1/3 of a letter grade deduction from the final grade on the major assignment for the module (for example, a B+ would become a B).

MODULE DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
T/W Feb 7/8 NLL
Lecture slides
Orientation and laboratory tour
M1D1 R/F Feb 9/10 AK
Complete in-silico cloning of protein expression plasmid
Orientation quiz
Homework due
M1D2 T/W Feb 14/15 AK
Perform protein purification protocol
Homework due
M1D3 R/F Feb 16/17 AK
Assess purity and concentration of purified protein
Homework due
T/W Feb 21/22 President's Day holiday
M1D4 R/F Feb 23/24 AK
Confirm purified protein using Western blot
Laboratory quiz
Homework due
M1D5 T/W Feb/Mar 28/1 AK
Image Western blot of purified protein
Homework due
M1D6 R/F Mar 2/3 AK
Prepare and scan small molecule microarray (SMM) slides
Homework due
Research talk due Sat, Mar 4 at 10pm
M1D7 T/W Mar 7/8 AK
Analyze SMM data to identify putative small molecule binders
Homework due
M1D8 R/F Mar 9/10 BE Comm Lab
Organize Data summary figures and results
Laboratory quiz
Homework due
M2D1 T/W Mar 14/15 BCM
Determine mutagenesis strategy
Homework due
M2D2 R/F Mar 16/17 BCM
Perform site-directed mutagenesis
Homework due
Data Summary draft due Sat, Mar 18 at 10 pm
Blog post due Mon, Mar 20 at 10 pm
M2D3 T/W Mar 21/22 BCM
Sequence clones and transform into yeast cells
Homework due
M2D4 R/F Mar 23/24 BCM
Determine transporter mutation and prepare metal uptake experiment
Laboratory quiz
Homework due
T/W Mar 28/29 - R/F Mar 30/31 Spring Break Data Summary revision due Mon, Mar 27 at 10 pm
T/W Apr 4/5 Journal article presentations
R/F Apr 6/7 Journal article presentations Blog post due Sat, Apr 8 at 10 pm
M2D5 T/W Apr 11/12 BCM
Analyze ICP-OES data
Homework due
M2D6 R/F Apr 13/14 BCM
IF staining and prepare for cytotoxicity experiment
Homework due
T/W Apr 18/19 Patriots' Day holiday
M2D7 R/F Apr 20/21 BCM
Confirm transporter expression and cell survival of yeast exposed to metal
Homework due
M2D8 T/W Apr 25/26 Comm Lab
Complete data analysis and organize Research article figures
Laboratory quiz
Homework due
M3D1 R/F Apr 27/28 BCM
Brainstorm ideas for Research proposal presentation
Homework due
M3D2 T/W May 2/3 AB
Pitch research proposal presentation ideas
Research article due Mon, May 1 at 10 pm
Blog post due Tue, May 2 at 10 pm
M3D3 R/F May 4/5 BE Comm Lab Develop ideas for Research proposal presentation
Homework due
M3D4 T/W May 9/10 NLL
Participate in Research proposal peer reviews
Homework due
R/F May 11/12 Research proposal presentations Blog post due Sat, May 13 at 10 pm
T May 16 Celebration lunch!