Difference between revisions of "20.109(S23):M1D7"
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==Protocols== | ==Protocols== | ||
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| + | ===Part 2: Complete by-eye analysis of hits identified in SMM using TDP43=== | ||
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| + | Using the analysis workflow described in Part 1 and the results of a biological assay completed by 109ers in Sp21 (ask your Instructor if you are interested in the details!), five small molecule compounds were selected for your research this semester. In this exercise you will evaluate the results and structures of the small molecules, then you will select which hits you want to test using functional assays. | ||
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| + | ====Evaluate raw data for hits==== | ||
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| + | Visually validate the raw data images for each of the small molecule compounds in the table above using the guidelines provided in Part 1. | ||
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| + | <font color = #4a9152 >'''In your laboratory notebook,'''</font color> complete the following: | ||
| + | *For each small molecule, answer the questions below: | ||
| + | **Is the z-score above the threshold value you define in Part 1? | ||
| + | **Do the images captured on the 532 nm channel appear as expected? Why or why not? | ||
| + | **Do the images captured on the 635 nm channel appear as expected? Why or why not? | ||
| + | **Are the images for the replicates consistent? | ||
==Reagents list== | ==Reagents list== | ||
Revision as of 20:38, 7 February 2023
Contents
Introduction
Protocols
Part 2: Complete by-eye analysis of hits identified in SMM using TDP43
Using the analysis workflow described in Part 1 and the results of a biological assay completed by 109ers in Sp21 (ask your Instructor if you are interested in the details!), five small molecule compounds were selected for your research this semester. In this exercise you will evaluate the results and structures of the small molecules, then you will select which hits you want to test using functional assays.
Evaluate raw data for hits
Visually validate the raw data images for each of the small molecule compounds in the table above using the guidelines provided in Part 1.
In your laboratory notebook, complete the following:
- For each small molecule, answer the questions below:
- Is the z-score above the threshold value you define in Part 1?
- Do the images captured on the 532 nm channel appear as expected? Why or why not?
- Do the images captured on the 635 nm channel appear as expected? Why or why not?
- Are the images for the replicates consistent?
Reagents list
Next day: Organize Data summary figures and results
