20.109(S23):Class data

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20.109(S23): Laboratory Fundamentals of Biological Engineering

Spring 2023 schedule FYI Assignments Homework Class data Communication Accessibility

M1: Drug discovery M2: Protein engineering M3: Project design

Contents

1 Module 1: Drug discovery
- 1.1 SDS-PAGE
  - 1.1.1 T/R
  - 1.1.2 W/F
- 1.2 BCA
  - 1.2.1 W/F
- 1.3 Western Blot
  - 1.3.1 W/F
- 1.4 SMM
2 Module 2: Protein engineering

Module 1: Drug discovery

SDS-PAGE

T/R

Red Team: Media:TR Red S23M1D3.jpg
Orange Team: Media:TR Orange S23M1D3.jpg
Yellow Team: Media:TR Yellow S23M1D3.jpg
Green Team: Media:TR Green S23M1D3.jpg
Blue Team: Media:TR Blue S23M1D3.jpg
Pink Team: Media:TR Pink S23M1D3.jpg
Purple Team: Media:TR Purple S23M1D3.jpg

W/F

Dropbox Link : https://www.dropbox.com/sh/1p3sfs2487pkwr0/AAAAA-W0W6vPcXcfcp2nV2TPa?dl=0

BCA

W/F

Media: Sp23 W F BCA.xlsx

Western Blot

W/F

Dropbox Link: https://www.dropbox.com/scl/fo/xlv4yz02oq2pfhka7mcmt/h?dl=0&rlkey=x1g0jqms054n0ycy3s2atbzrf

SMM

Module 2: Protein engineering

Mutagenesis

T/R

Team	Mutation (eg. S301L)	Forward primer	Reverse primer
TR red
TR orange
TR yellow
TR green
TR blue
TR pink
TR purple	Y276C	TGGCAGATGTgTATTAACACTG	AGCGTCACTGAACTTTTTC

W/F

Team	Mutation (eg. S301L)	Forward primer	Reverse primer
WF red
WF orange
WF yellow
WF green
WF blue
WF pink
WF purple

ICP-OES data

T/R

Raw ICP-OES data [here]

Team	Calculated Fe uptake	Calculated Cd update	Data file
TR red
TR orange
TR yellow
TR green
TR blue
TR pink
TR purple

W/F

Raw ICP-OES data [here]

Team	Calculated Fe uptake	Calculated Cd update	Data file
WF red
WF orange
WF yellow
WF green
WF blue
WF pink
WF purple

Cytotoxicity data

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20.109ExportS23