Difference between revisions of "20.109(S23):Class data"
From Course Wiki
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|TR red | |TR red | ||
| − | | | + | | 2.62 ppm / 240.489 nm |
| − | | | + | | 7.58 ppm / 361.287 nm |
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Revision as of 19:39, 20 April 2023
Contents
Module 1: Drug discovery
SDS-PAGE
T/R
Red Team: Media:TR Red S23M1D3.jpg
Orange Team: Media:TR Orange S23M1D3.jpg
Yellow Team: Media:TR Yellow S23M1D3.jpg
Green Team: Media:TR Green S23M1D3.jpg
Blue Team: Media:TR Blue S23M1D3.jpg
Pink Team: Media:TR Pink S23M1D3.jpg
Purple Team: Media:TR Purple S23M1D3.jpg
W/F
Dropbox Link : https://www.dropbox.com/sh/1p3sfs2487pkwr0/AAAAA-W0W6vPcXcfcp2nV2TPa?dl=0
BCA
W/F
Western Blot
W/F
Dropbox Link: https://www.dropbox.com/scl/fo/xlv4yz02oq2pfhka7mcmt/h?dl=0&rlkey=x1g0jqms054n0ycy3s2atbzrf
SMM
Module 2: Protein engineering
Mutagenesis
T/R
| Team | Mutation (eg. S301L) | Forward primer | Reverse primer |
| TR red | Y276C | TGGCAGATGTgTATTAACACTG | AGCGTCACTGAACTTTTTC |
| TR orange | Y276C | TTGGCAGATGtgtATTAACACTG | GCGTCACTGAACTTTTTC |
| TR yellow | Y276C | GGCAGATGTAcATTAACACTG | AAGCGTCACTGAACTTTTTC |
| TR green | Y352C | GATTGATTGGtgtGCTGATATTATTG | ATCTTTCTCCCTGTCGAC |
| TR blue | Y276C | TGGCAGATGTgTATTAACACTG | AGCGTCACTGAACTTTTTC |
| TR pink | D354C | TTGGTATGCTtgtATTATTGGTACTGG | TCAATCATCTTTCTCCCTG |
| TR purple | Y276C | TGGCAGATGTgTATTAACACTG | AGCGTCACTGAACTTTTTC |
T/R Sequencing [here]
W/F
| Team | Mutation (eg. S301L) | Forward primer | Reverse primer |
| WF red | V281C | TAACACTGCAtgTGCAGTTTCCCTTC | ATATACATCTGCCAAGCG |
| WF orange | I349C | GAGAAAGATGtgTGATTGGTATGCTGATATTATTG | CCTGTCGACCTTTTTTTAG |
| WF yellow | R405C | TTTCGTTTTGtgtGAAGTGTACTTCCG | CCGTCCAAAAACCCAATTAAAC |
| WF green | D271C | AAAGTTCAGTTGCGCTTGGCAGATGTATATTAACACTGCAGTTGC (AAAGTTCAGTtgcGCTTGGCAGATG) | TTCAATCTTGGATTACTACCTGTAGTTTCTCCGGTGTAAGGTGGA (TTCAATCTTGGATTACTACCTG) |
| WF blue | Y408C | ||
| WF pink | D400C | ATTGGGTTTTTGtgCGGTTTCGTTTTG (TGGGTTTTTGtgcGGTTTCGTTTTG) | TAAACCTGTGTATGTACCGATAATCAACCACC (ATTAAACCTGTGTATGTACC) |
| WF purple | D350C | AAAGATGATTtgtTGGTATGCTGATATTATTGGTAC | CTCCCTGTCGACCTTTTTTTAG |
WF Sequencing here
Immunofluorescence Data
Pilot Immunofluorescence Data [[1]]
ICP-OES data
T/R
Raw ICP-OES data [here]
| Team | Fe (ppm)/ Wavelength | Cd (ppm)/ Wavelength | O.D. |
| TR red | 2.62 ppm / 240.489 nm | 7.58 ppm / 361.287 nm | |
| TR orange | |||
| TR yellow | 11.75 ppm / 373.713 nm | 11.35 ppm / 346.766 nm | 1.529 |
| TR green | 11.91 ppm / 373.713 nm | 11.50 ppm / 479.991 nm | 1.495 |
| TR blue | 4.66 ppm / 259.837 nm | 11.52 ppm /479.999 nm | 1.546 |
| TR pink | 4.03ppm / 238.863nm | 10.52ppm / 232.929nm | 1.581 |
| TR purple | |||
| TR WT | 1 | ||
| TR Fet4 | 1.28 |
W/F
Raw ICP-OES data [here]
| Team | Fe (ppm)/ Wavelength | Cd (ppm)/ Wavelength | O.D. |
| WF red | |||
| WF orange | |||
| WF yellow | |||
| WF green | |||
| WF blue | |||
| WF pink | |||
| WF purple | |||
| WF WT | 1.18 | ||
| WF Fet4 | 1.4 |
