Difference between revisions of "20.109(S23):Class data"

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Revision as of 20:01, 15 March 2023

20.109(S23): Laboratory Fundamentals of Biological Engineering

Sp23 banner image v2.png

Spring 2023 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       M1: Drug discovery        M2: Protein engineering        M3: Project design       


Module 1: Drug discovery

SDS-PAGE

T/R

Red Team: Media:TR Red S23M1D3.jpg
Orange Team: Media:TR Orange S23M1D3.jpg
Yellow Team: Media:TR Yellow S23M1D3.jpg
Green Team: Media:TR Green S23M1D3.jpg
Blue Team: Media:TR Blue S23M1D3.jpg
Pink Team: Media:TR Pink S23M1D3.jpg
Purple Team: Media:TR Purple S23M1D3.jpg

W/F

Dropbox Link : https://www.dropbox.com/sh/1p3sfs2487pkwr0/AAAAA-W0W6vPcXcfcp2nV2TPa?dl=0

BCA

W/F

Media: Sp23 W F BCA.xlsx

Western Blot

W/F

Dropbox Link: https://www.dropbox.com/scl/fo/xlv4yz02oq2pfhka7mcmt/h?dl=0&rlkey=x1g0jqms054n0ycy3s2atbzrf

SMM

SMM results

Module 2: Protein engineering

Mutagenesis

T/R

Team Mutation (eg. S301L) Forward primer Reverse primer
TR red Y276C TGGCAGATGTgTATTAACACTG AGCGTCACTGAACTTTTTC
TR orange Y276C TTGGCAGATGtgtATTAACACTG GCGTCACTGAACTTTTTC
TR yellow Y276C GGCAGATGTAcATTAACACTG AAGCGTCACTGAACTTTTTC
TR green Y352C GATTGATTGGtgtGCTGATATTATTG ATCTTTCTCCCTGTCGAC
TR blue Y276C TGGCAGATGTgTATTAACACTG AGCGTCACTGAACTTTTTC
TR pink D354C TTGGTATGCTtgtATTATTGGTACTGG TCAATCATCTTTCTCCCTG
TR purple Y276C TGGCAGATGTgTATTAACACTG AGCGTCACTGAACTTTTTC

W/F

Team Mutation (eg. S301L) Forward primer Reverse primer
WF red V281C TAACACTGCAtgTGCAGTTTCCCTTC ATATACATCTGCCAAGCG
WF orange
WF yellow
WF green
WF blue
WF pink
WF purple

ICP-OES data

T/R

Raw ICP-OES data [here]

Team Calculated Fe uptake Calculated Cd update Data file
TR red
TR orange
TR yellow
TR green
TR blue
TR pink
TR purple

W/F

Raw ICP-OES data [here]

Team Calculated Fe uptake Calculated Cd update Data file
WF red
WF orange
WF yellow
WF green
WF blue
WF pink
WF purple

Cytotoxicity data

[TR section]

[WF section]
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