Difference between revisions of "20.109(S22):Class data"

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(→‎T/R)
(→‎small molecule selections)
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|'''small molecule #1 (enter compound ID)'''
 
|'''small molecule #1 (enter compound ID)'''
 
|'''small molecule #2 (enter compound ID)'''
 
|'''small molecule #2 (enter compound ID)'''
 +
|'''aggregation assay results'''
 
|-
 
|-
 
| TR Red
 
| TR Red
 
| 83079118
 
| 83079118
 
| 69269200
 
| 69269200
 +
|
 
|-
 
|-
 
| TR Orange
 
| TR Orange
 
| 95877382
 
| 95877382
 
| 83023303
 
| 83023303
 +
|
 
|-
 
|-
 
| TR Yellow
 
| TR Yellow
 
|  
 
|  
 
|  
 
|  
 +
|
 
|-
 
|-
 
| TR Green
 
| TR Green
 
|69269200
 
|69269200
 
|83079118  
 
|83079118  
 +
|
 
|-
 
|-
 
| TR Blue
 
| TR Blue
 
|69269200
 
|69269200
 
|83079118  
 
|83079118  
−
|  
+
|
 
|-
 
|-
 
| TR Teal
 
| TR Teal
 
|69269200
 
|69269200
 
|83079118  
 
|83079118  
 +
|
 
|-
 
|-
 
| TR Pink
 
| TR Pink
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| 69269200
 
| 69269200
 
| 83079118
 
| 83079118
 +
|
 
|-
 
|-
 
| TR Grey
 
| TR Grey
 
|69269200
 
|69269200
 
|83079118  
 
|83079118  
 +
|
 
|-
 
|-
 
| TR White
 
| TR White
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| 69269200
 
| 69269200
 
| 83079118
 
| 83079118
 +
|
 
|-
 
|-
 
| WF Orange
 
| WF Orange
 
| 83079118
 
| 83079118
 
| 69269200
 
| 69269200
 +
|
 
|-
 
|-
 
| WF Yellow
 
| WF Yellow
 
| 83079118
 
| 83079118
 
| 69269200
 
| 69269200
 +
|
 
|-
 
|-
 
| WF Green
 
| WF Green
 
|83079118
 
|83079118
 
|83023303
 
|83023303
 +
|
 
|-
 
|-
 
| WF Blue
 
| WF Blue
 
| 69269200  
 
| 69269200  
 
| 83079118  
 
| 83079118  
−
|-
 
−
| WF Teal
 
−
|
 
 
|
 
|
 
|-
 
|-
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| 83079118
 
| 83079118
 
| 69269200
 
| 69269200
−
 
 
|
 
|
 
|-
 
|-
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| 69269200
 
| 69269200
 
| 83079118
 
| 83079118
−
|-
 
−
| WF Grey
 
−
|
 
 
|
 
|
 
|-
 
|-
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| 69269200
 
| 69269200
 
| 83023303
 
| 83023303
 +
|
 
|}
 
|}
  

Revision as of 18:47, 14 February 2022

20.109(S22): Laboratory Fundamentals of Biological Engineering

Sp17 20.109 M1D7 chemical structure features.png

Spring 2022 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       M1: Drug discovery        M2: Metabolic engineering        M3: Project design       


Module 1: Drug discovery

small molecule selections

T/R

Team small molecule #1 (enter compound ID) small molecule #2 (enter compound ID) aggregation assay results
TR Red 83079118 69269200
TR Orange 95877382 83023303
TR Yellow
TR Green 69269200 83079118
TR Blue 69269200 83079118
TR Teal 69269200 83079118
TR Pink 69269200 83079118
TR Purple 69269200 83079118
TR Grey 69269200 83079118
TR White 69269200 83079118

W/F

Team small molecule #1 (enter compound ID) small molecule #2 (enter compound ID)
WF Red 69269200 83079118
WF Orange 83079118 69269200
WF Yellow 83079118 69269200
WF Green 83079118 83023303
WF Blue 69269200 83079118
WF Pink 83079118 69269200
WF Purple 69269200 83079118
WF White 69269200 83023303

Module 2: Metabolic engineering

sgRNA_target sequences

T/R

Team Ethanol (E) or Acetate (A) Gene targeted by CRISPRi gRNA gRNA (DNA) sequence (without tag at 3' end) Locus targeted (eg. beginning of gene, putative promoter, -35 region) Target coding or non-coding strand Ethanol / Acetate Assay Results
TR Red
TR Orange
TR Yellow
TR Green
TR Blue
TR Teal
TR Pink
TR Purple
TR Grey
TR White

W/F

Team Ethanol (E) or Acetate (A) Gene targeted by CRISPRi gRNA gRNA (DNA) sequence (without tag at 3' end) Locus targeted (eg. beginning of gene, putative promoter, -35 region) Target coding or non-coding strand Colorimetric Assay Results
WF Red
WF Orange
WF Yellow
WF Green
WF Blue
WF Teal
WF Pink
WF Purple
WF Grey
WF White
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