Difference between revisions of "20.109(S22):Class data"
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Revision as of 13:30, 23 February 2022
Contents
Module 1: Drug discovery
SDS-PAGE gel images
small molecule selections
T/R
| Team | small molecule #1 (enter compound ID) | small molecule #2 (enter compound ID) | aggregation assay results |
| TR Red | 83079118 | 69269200 | https://www.dropbox.com/s/7eut5ymxmiel9rp/TR_teal_red.xlsx?dl=0 |
| TR Orange | 95877382 | 83023303 | https://docs.google.com/spreadsheets/d/1Lr5QAYY-63bOfrxuzZh1hgpHfTkCcTuU/edit?usp=sharing&ouid=103020115084669070409&rtpof=true&sd=true |
| TR Yellow | 69269200 | 83079118 | |
| TR Green | 69269200 | 83079118 | |
| TR Blue | 69269200 | 83079118 | |
| TR Teal | 69269200 | 83079118 | https://www.dropbox.com/scl/fi/1cs77ilahj51l51cw0j2g/TR_Teal_83079119.xlsx?dl=0&rlkey=8l1cae481k6p7p5y8290e8pu2 |
| TR Pink | 69269200 | 83079118 | |
| TR Purple | 69269200 | 83079118 | |
| TR Grey | 69269200 | 83079118 | |
| TR White | 69269200 | 83079118 |
W/F
| Team | small molecule #1 (enter compound ID) | small molecule #2 (enter compound ID) | aggregation assay results | |
| WF Red | 69269200 | 83079118 | https://mitprod-my.sharepoint.com/:x:/g/personal/mrowlett_mit_edu/EXXtvBLjG9BLtZajiE1PqbUBBkSUVd5xolqzA2KO1u1ADg?e=JjooPh | |
| WF Orange | 83079118 | 69269200 | ||
| WF Yellow | 83079118 | 69269200 | ||
| WF Green | 83079118 | 83023303 | ||
| WF Blue | 69269200 | 83079118 | https://www.dropbox.com/s/7gf6imiiuofur37/20220218_agg_assay_blue%20%2883079118%29.xlsx?dl=0 | |
| WF Pink | 83079118 | 69269200 | ||
| WF Purple | 69269200 | 83079118 | ||
| WF White | 69269200 | 83023303 |
Aggregation Results
[T/R Aggregation dropbox folder]
Module 2: Metabolic engineering
sgRNA_target sequences
T/R
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA (DNA) sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target coding or non-coding strand | Ethanol / Acetate Assay Results |
| TR Red | ||||||
| TR Orange | ||||||
| TR Yellow | ||||||
| TR Green | ||||||
| TR Blue | ||||||
| TR Teal | ||||||
| TR Pink | ||||||
| TR Purple | ||||||
| TR Grey | ||||||
| TR White |
W/F
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA (DNA) sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target coding or non-coding strand | Colorimetric Assay Results |
| WF Red | ||||||
| WF Orange | ||||||
| WF Yellow | ||||||
| WF Green | ||||||
| WF Blue | ||||||
| WF Teal | ||||||
| WF Pink | ||||||
| WF Purple | ||||||
| WF Grey | ||||||
| WF White |
