Difference between revisions of "20.109(S22):Class data"

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(gRNA sequences)
(W/F)
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|'''small molecule #2 (enter compound ID)'''
 
|'''small molecule #2 (enter compound ID)'''
 
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| TR Red
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| WF Red
 
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| TR Orange
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| WF Orange
 
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| TR Yellow
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| WF Yellow
 
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| TR Green
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| WF Green
 
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| TR Blue
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| WF Blue
 
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| TR Teal
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| WF Teal
 
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| TR Pink
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| WF Pink
 
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| TR Purple
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| WF Purple
 
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| TR Grey
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| WF Grey
 
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| TR White
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| WF White
 
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Revision as of 19:33, 27 January 2022

20.109(S22): Laboratory Fundamentals of Biological Engineering

Sp17 20.109 M1D7 chemical structure features.png

Spring 2022 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       M1: Drug discovery        M2: Metabolic engineering        M3: Project design       


Module 1: Drug discovery

small molecule selections

T/R

Team small molecule #1 (enter compound ID) small molecule #2 (enter compound ID)
TR Red
TR Orange
TR Yellow
TR Green
TR Blue
TR Teal
TR Pink
TR Purple
TR Grey
TR White

W/F

Team small molecule #1 (enter compound ID) small molecule #2 (enter compound ID)
WF Red
WF Orange
WF Yellow
WF Green
WF Blue
WF Teal
WF Pink
WF Purple
WF Grey
WF White

Module 2: Metabolic engineering

sgRNA_target sequences

T/R

Team Ethanol (E) or Acetate (A) Gene targeted by CRISPRi gRNA gRNA (DNA) sequence (without tag at 3' end) Locus targeted (eg. beginning of gene, putative promoter, -35 region) Target coding or non-coding strand Ethanol / Acetate Assay Results
TR Red
TR Orange
TR Yellow
TR Green
TR Blue
TR Teal
TR Pink
TR Purple
TR Grey
TR White

W/F

Team Ethanol (E) or Acetate (A) Gene targeted by CRISPRi gRNA gRNA (DNA) sequence (without tag at 3' end) Locus targeted (eg. beginning of gene, putative promoter, -35 region) Target coding or non-coding strand Colorimetric Assay Results
WF Red
WF Orange
WF Yellow
WF Green
WF Blue
WF Teal
WF Pink
WF Purple
WF Grey
WF White