Difference between revisions of "20.109(S20): Spring 2020 schedule"
From Course Wiki
Noreen Lyell (Talk | contribs) |
Noreen Lyell (Talk | contribs) |
||
| Line 19: | Line 19: | ||
| | | | ||
| | | | ||
| − | | T/W Feb | + | | T/W Feb 4/5 |
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | ||
| [[20.109(S20):Laboratory tour | Orientation and laboratory tour]] | | [[20.109(S20):Laboratory tour | Orientation and laboratory tour]] | ||
| Line 28: | Line 28: | ||
| 1 | | 1 | ||
| 1 | | 1 | ||
| − | | R/F Feb | + | | R/F Feb 6/7 |
| [http://be.mit.edu/directory/angela-koehler AK]<br> | | [http://be.mit.edu/directory/angela-koehler AK]<br> | ||
| [[20.109(S20):In silico cloning and confirmation digest of protein expression vector(Day1) |In silico cloning and confirmation digest of protein expression vector]]<br> | | [[20.109(S20):In silico cloning and confirmation digest of protein expression vector(Day1) |In silico cloning and confirmation digest of protein expression vector]]<br> | ||
| Line 35: | Line 35: | ||
| 1 | | 1 | ||
| 2 | | 2 | ||
| − | | T/W Feb | + | | T/W Feb 11/12 |
| [http://be.mit.edu/directory/angela-koehler AK]<br> | | [http://be.mit.edu/directory/angela-koehler AK]<br> | ||
| [[20.109(S20):Purify protein for secondary assays(Day2) |Purify protein for secondary assays]]<br> | | [[20.109(S20):Purify protein for secondary assays(Day2) |Purify protein for secondary assays]]<br> | ||
| Line 42: | Line 42: | ||
| 1 | | 1 | ||
| 3 | | 3 | ||
| − | | R/F Feb | + | | R/F Feb 13/14 |
| [http://be.mit.edu/directory/angela-koehler AK]<br> | | [http://be.mit.edu/directory/angela-koehler AK]<br> | ||
| [[20.109(S20):Analyze small microarray data (Day3) | Analyze small molecule microarray (SMM) data]]<br> | | [[20.109(S20):Analyze small microarray data (Day3) | Analyze small molecule microarray (SMM) data]]<br> | ||
| Line 49: | Line 49: | ||
| | | | ||
| | | | ||
| − | | T/W Feb | + | | T/W Feb 18/19 |
| | | | ||
| <font color = #FACC2E>'''President's day holiday'''</font color> | | <font color = #FACC2E>'''President's day holiday'''</font color> | ||
| Line 56: | Line 56: | ||
| 1 | | 1 | ||
| 4 | | 4 | ||
| − | | R/F Feb | + | | R/F Feb 20/21 |
| [http://be.mit.edu/directory/angela-koehler AK]<br> | | [http://be.mit.edu/directory/angela-koehler AK]<br> | ||
| [[20.109(S20):Evaluate protein purity and concentration (Day4) |Evaluate protein purity and concentration]]<br> | | [[20.109(S20):Evaluate protein purity and concentration (Day4) |Evaluate protein purity and concentration]]<br> | ||
| Line 63: | Line 63: | ||
| 1 | | 1 | ||
| 5 | | 5 | ||
| − | | T/W Feb | + | | T/W Feb 25/26 |
| [http://be.mit.edu/directory/angela-koehler AK]<br> | | [http://be.mit.edu/directory/angela-koehler AK]<br> | ||
| [[20.109(S20):Test protein activity using peptidyl-prolyl cis-trans isomerase assay (Day5) |Test protein activity using peptidyl-prolyl cis-trans isomerase assay]]<br> | | [[20.109(S20):Test protein activity using peptidyl-prolyl cis-trans isomerase assay (Day5) |Test protein activity using peptidyl-prolyl cis-trans isomerase assay]]<br> | ||
| Line 70: | Line 70: | ||
| 1 | | 1 | ||
| 6 | | 6 | ||
| − | | R/F Feb/ | + | | R/F Feb 27/28 |
| [http://be.mit.edu/directory/angela-koehler AK]<br> | | [http://be.mit.edu/directory/angela-koehler AK]<br> | ||
| [[20.109(S20):Confirm ligand binding using differential scanning fluorimetry assay (Day6) |Confirm ligand binding using differential scanning fluorimetry assay]]<br> | | [[20.109(S20):Confirm ligand binding using differential scanning fluorimetry assay (Day6) |Confirm ligand binding using differential scanning fluorimetry assay]]<br> | ||
| Line 77: | Line 77: | ||
| 1 | | 1 | ||
| 7 | | 7 | ||
| − | | T/W Mar | + | | T/W Mar 3/4 |
| [http://be.mit.edu/directory/angela-koehler AK]<br> | | [http://be.mit.edu/directory/angela-koehler AK]<br> | ||
| [[20.109(S20):Complete data analysis (Day7) |Complete data analysis]]<br> | | [[20.109(S20):Complete data analysis (Day7) |Complete data analysis]]<br> | ||
| Line 86: | Line 86: | ||
| 2 | | 2 | ||
| 1 | | 1 | ||
| − | | R/F Mar | + | | R/F Mar 5/6 |
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | ||
| [[20.109(S20):Practice tissue culture techniques and prepare cells for RNA purification (Day1)| Practice tissue culture techniques and prepare cells for RNA purification]]<br> | | [[20.109(S20):Practice tissue culture techniques and prepare cells for RNA purification (Day1)| Practice tissue culture techniques and prepare cells for RNA purification]]<br> | ||
| Line 93: | Line 93: | ||
| 2 | | 2 | ||
| 2 | | 2 | ||
| − | | T/W Mar | + | | T/W Mar 10/11 |
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | ||
| [[20.109(S20):Etoposide treat cells for RNA purification (Day2)| Etoposide treat cells for RNA purification]]<br> | | [[20.109(S20):Etoposide treat cells for RNA purification (Day2)| Etoposide treat cells for RNA purification]]<br> | ||
| Line 100: | Line 100: | ||
| 2 | | 2 | ||
| 3 | | 3 | ||
| − | | R/F Mar | + | | R/F Mar 12/13 |
| [http://be.mit.edu/directory/ernest-fraenkel EF] <br> | | [http://be.mit.edu/directory/ernest-fraenkel EF] <br> | ||
| [[20.109(S20):Purify RNA and practice RNA-seq data analysis methods (Day3)| Purify RNA and practice RNA-seq data analysis methods]]<br> | | [[20.109(S20):Purify RNA and practice RNA-seq data analysis methods (Day3)| Purify RNA and practice RNA-seq data analysis methods]]<br> | ||
| Line 107: | Line 107: | ||
| 2 | | 2 | ||
| 4 | | 4 | ||
| − | | T/W Mar | + | | T/W Mar 17/18 |
| [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | ||
| [[20.109(S20):Analyze RNA-seq data and prepare for quantitative PCR experiment (Day 4)| Analyze RNA-seq data and prepare for quantitative PCR experiment]]<br> | | [[20.109(S20):Analyze RNA-seq data and prepare for quantitative PCR experiment (Day 4)| Analyze RNA-seq data and prepare for quantitative PCR experiment]]<br> | ||
| Line 114: | Line 114: | ||
| 2 | | 2 | ||
| 5 | | 5 | ||
| − | | R/F Mar | + | | R/F Mar 19/20 |
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | ||
| [[20.109(S20):Perform quantitative PCR experiment and explore additional RNA-seq datasets (Day5) | Perform quantitative PCR experiment and explore additional RNA-seq datasets]]<br> | | [[20.109(S20):Perform quantitative PCR experiment and explore additional RNA-seq datasets (Day5) | Perform quantitative PCR experiment and explore additional RNA-seq datasets]]<br> | ||
| Line 121: | Line 121: | ||
| | | | ||
| | | | ||
| − | | T/W Mar | + | | T/W Mar 24/25 |
| | | | ||
| <font color = #FACC2E>'''Spring vacation'''</font color> | | <font color = #FACC2E>'''Spring vacation'''</font color> | ||
| Line 128: | Line 128: | ||
| | | | ||
| | | | ||
| − | | R/F Mar | + | | R/F Mar 26/27 |
| | | | ||
| <font color = #FACC2E>'''Spring vacation'''</font color> | | <font color = #FACC2E>'''Spring vacation'''</font color> | ||
| Line 135: | Line 135: | ||
| 2 | | 2 | ||
| 6 | | 6 | ||
| − | | T/W Apr | + | | T/W Mar/Apr 31/1 |
| [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | ||
| [[20.109(S20):Design cell viability experiment and complete RNA-seq data analysis (Day6)| Design cell viability experiment and complete RNA-seq data analysis]]<br> | | [[20.109(S20):Design cell viability experiment and complete RNA-seq data analysis (Day6)| Design cell viability experiment and complete RNA-seq data analysis]]<br> | ||
| Line 142: | Line 142: | ||
| 2 | | 2 | ||
| 7 | | 7 | ||
| − | | R/F Apr | + | | R/F Apr 2/3 |
| Comm Lab<br> | | Comm Lab<br> | ||
| [[20.109(S20):Journal club presentations (Day7 and 8)| Journal club I presentations]]<br> | | [[20.109(S20):Journal club presentations (Day7 and 8)| Journal club I presentations]]<br> | ||
| Line 149: | Line 149: | ||
| 2 | | 2 | ||
| 8 | | 8 | ||
| − | | T/W Apr | + | | T/W Apr 7/8 |
| [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | ||
| [[20.109(S20):Journal club presentations (Day7 and 8)| Journal club II presentations]]<br> | | [[20.109(S20):Journal club presentations (Day7 and 8)| Journal club II presentations]]<br> | ||
| | | | ||
|-- | |-- | ||
| − | | | + | | colspan="7" | |
| − | | | + | |-- |
| − | | R/F Apr | + | | 3 |
| + | | 1 | ||
| + | | R/F Apr 9/10 | ||
| [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | ||
| [[20.109(S20):Complete data analysis (Day9)| Complete data analysis]]<br> | | [[20.109(S20):Complete data analysis (Day9)| Complete data analysis]]<br> | ||
| <font color= #00BFFF>'''Laboratory quiz'''</font color> <br> [[20.109(S20):Homework#Due_M2D9| Homework due]] <br> | | <font color= #00BFFF>'''Laboratory quiz'''</font color> <br> [[20.109(S20):Homework#Due_M2D9| Homework due]] <br> | ||
| − | |||
| − | |||
|-- | |-- | ||
| | | | ||
Revision as of 19:23, 14 January 2020
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm (32-124)
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm (56-322)
