Difference between revisions of "20.109(S20): Spring 2020 schedule"
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Noreen Lyell (Talk | contribs) |
Noreen Lyell (Talk | contribs) |
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| Line 20: | Line 20: | ||
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| T/W Feb 5/6 | | T/W Feb 5/6 | ||
| − | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Laboratory tour | Orientation and laboratory tour]] |
| | | | ||
|-- | |-- | ||
| Line 29: | Line 29: | ||
| 1 | | 1 | ||
| R/F Feb 7/8 | | R/F Feb 7/8 | ||
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br> | + | | [http://be.mit.edu/directory/angela-koehler AK]<br> |
| − | | [[20.109( | + | | [[20.109(S20):In silico cloning and confirmation digest of protein expression vector(Day1) |In silico cloning and confirmation digest of protein expression vector]]<br> |
| − | | <font color= # | + | | <font color= #00BFFF>'''Orientation quiz'''</font color> <br> [[20.109(S20):Homework#Due_M1D1| Homework due]] |
|-- | |-- | ||
| 1 | | 1 | ||
| 2 | | 2 | ||
| T/W Feb 12/13 | | T/W Feb 12/13 | ||
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br> | + | | [http://be.mit.edu/directory/angela-koehler AK]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Purify protein for secondary assays(Day2) |Purify protein for secondary assays]]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Homework#Due_M1D2| Homework due]] |
|-- | |-- | ||
| 1 | | 1 | ||
| 3 | | 3 | ||
| R/F Feb 14/15 | | R/F Feb 14/15 | ||
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br> | + | | [http://be.mit.edu/directory/angela-koehler AK]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Analyze small microarray data (Day3) | Analyze small molecule microarray (SMM) data]]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Homework#Due_M1D3| Homework due]] |
|-- | |-- | ||
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| Line 57: | Line 57: | ||
| 4 | | 4 | ||
| R/F Feb 21/22 | | R/F Feb 21/22 | ||
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br> | + | | [http://be.mit.edu/directory/angela-koehler AK]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Evaluate protein purity and concentration (Day4) |Evaluate protein purity and concentration]]<br> |
| − | | <font color= # | + | | <font color= #00BFFF>'''Laboratory quiz'''</font color> <br> [[20.109(S20):Homework#Due_M1D4| Homework due]] |
|-- | |-- | ||
| 1 | | 1 | ||
| 5 | | 5 | ||
| T/W Feb 26/27 | | T/W Feb 26/27 | ||
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br> | + | | [http://be.mit.edu/directory/angela-koehler AK]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Test protein activity using peptidyl-prolyl cis-trans isomerase assay (Day5) |Test protein activity using peptidyl-prolyl cis-trans isomerase assay]]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Homework#Due_M1D5| Homework due]] |
|-- | |-- | ||
| 1 | | 1 | ||
| 6 | | 6 | ||
| R/F Feb/Mar 28/1 | | R/F Feb/Mar 28/1 | ||
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br> | + | | [http://be.mit.edu/directory/angela-koehler AK]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Confirm ligand binding using differential scanning fluorimetry assay (Day6) |Confirm ligand binding using differential scanning fluorimetry assay]]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Homework#Due_M1D6| Homework due]] |
|-- | |-- | ||
| 1 | | 1 | ||
| 7 | | 7 | ||
| T/W Mar 5/6 | | T/W Mar 5/6 | ||
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br> | + | | [http://be.mit.edu/directory/angela-koehler AK]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Complete data analysis (Day7) |Complete data analysis]]<br> |
| − | | <font color= # | + | | <font color= #00BFFF>'''Laboratory quiz'''</font color> <br> [[20.109(S20):Homework#Due_M1D7| Homework due]] |
|-- | |-- | ||
| colspan="7" | | | colspan="7" | | ||
| Line 87: | Line 87: | ||
| 1 | | 1 | ||
| R/F Mar 7/8 | | R/F Mar 7/8 | ||
| − | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Practice tissue culture techniques and prepare cells for RNA purification (Day1)| Practice tissue culture techniques and prepare cells for RNA purification]]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Homework#Due_M2D1| Homework due]] |
|-- | |-- | ||
| 2 | | 2 | ||
| 2 | | 2 | ||
| T/W Mar 12/13 | | T/W Mar 12/13 | ||
| − | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Etoposide treat cells for RNA purification (Day2)| Etoposide treat cells for RNA purification]]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Homework#Due_M2D2| Homework due]]<br> [[20.109(S20): Data Summary| <font color=#FF0000>Data summary draft due</font color>]] Mon, Mar 11 at 10 pm<br> |
|-- | |-- | ||
| 2 | | 2 | ||
| 3 | | 3 | ||
| R/F Mar 14/15 | | R/F Mar 14/15 | ||
| − | | [http://be.mit.edu/directory/ernest-fraenkel EF] <br> | + | | [http://be.mit.edu/directory/ernest-fraenkel EF] <br> |
| − | | [[20.109( | + | | [[20.109(S20):Purify RNA and practice RNA-seq data analysis methods (Day3)| Purify RNA and practice RNA-seq data analysis methods]]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Homework#Due_M2D3| Homework due]] <br> [[20.109(S20): Mini-presentation| <font color=#FF0000>Mini-presentation due</font color>]] Sat, Mar 16 at 10 pm<br>[https:// Blog post due] Sun, Mar 17 at 10 pm <br> |
|-- | |-- | ||
| 2 | | 2 | ||
| 4 | | 4 | ||
| T/W Mar 19/20 | | T/W Mar 19/20 | ||
| − | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | + | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Analyze RNA-seq data and prepare for quantitative PCR experiment (Day 4)| Analyze RNA-seq data and prepare for quantitative PCR experiment]]<br> |
| − | | <font color= # | + | | <font color= #00BFFF>'''Laboratory quiz'''</font color> <br> [[20.109(S20):Homework#Due_M2D4| Homework due]] |
|-- | |-- | ||
| 2 | | 2 | ||
| 5 | | 5 | ||
| R/F Mar 21/22 | | R/F Mar 21/22 | ||
| − | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Perform quantitative PCR experiment and explore additional RNA-seq datasets (Day5) | Perform quantitative PCR experiment and explore additional RNA-seq datasets]]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Homework#Due_M2D5| Homework due]] |
|-- | |-- | ||
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| <font color = #FACC2E>'''Spring vacation'''</font color> | | <font color = #FACC2E>'''Spring vacation'''</font color> | ||
| − | | [[20.109( | + | | [[20.109(S20): Data Summary| <font color=#FF0000>Data summary revision due</font color>]] Mon, Mar 25 at 10 pm |
|-- | |-- | ||
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| Line 136: | Line 136: | ||
| 6 | | 6 | ||
| T/W Apr 2/3 | | T/W Apr 2/3 | ||
| − | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | + | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Design cell viability experiment and complete RNA-seq data analysis (Day6)| Design cell viability experiment and complete RNA-seq data analysis]]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Homework#Due_M2D6| Homework due]] |
|-- | |-- | ||
| 2 | | 2 | ||
| Line 144: | Line 144: | ||
| R/F Apr 4/5 | | R/F Apr 4/5 | ||
| Comm Lab<br> | | Comm Lab<br> | ||
| − | | [[20.109( | + | | [[20.109(S20):Journal club presentations (Day7 and 8)| Journal club I presentations]]<br> |
| − | | [https:/ | + | | [https:// Blog post due] Sat, Apr 6 at 10 pm <br> |
|-- | |-- | ||
| 2 | | 2 | ||
| Line 151: | Line 151: | ||
| T/W Apr 9/10 | | T/W Apr 9/10 | ||
| [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | ||
| − | | [[20.109( | + | | [[20.109(S20):Journal club presentations (Day7 and 8)| Journal club II presentations]]<br> |
| | | | ||
|-- | |-- | ||
| Line 158: | Line 158: | ||
| R/F Apr 11/12 | | R/F Apr 11/12 | ||
| [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | ||
| − | | [[20.109( | + | | [[20.109(S20):Complete data analysis (Day9)| Complete data analysis]]<br> |
| − | | <font color= # | + | | <font color= #00BFFF>'''Laboratory quiz'''</font color> <br> [[20.109(S20):Homework#Due_M2D9| Homework due]] <br> |
|-- | |-- | ||
| colspan="7" | | | colspan="7" | | ||
| Line 173: | Line 173: | ||
| 1 | | 1 | ||
| R/F Apr 18/19 | | R/F Apr 18/19 | ||
| − | | [http://be.mit.edu/directory/angela-belcher AB]<br> | + | | [http://be.mit.edu/directory/angela-belcher AB]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Growth of phage-based active (cathode) material (Day1) | Grow phage-based active (cathode) material]]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Homework#Due_M3D1| Homework due]] <br> [[20.109(S20): Research article| <font color=#FF0000>Research article due</font color>]] Sat, Apr 20 at 10 pm <br> [https:// Blog post due] Sun, Apr 21 at 10 pm |
|-- | |-- | ||
| 3 | | 3 | ||
| 2 | | 2 | ||
| T/W Apr 23/24 | | T/W Apr 23/24 | ||
| − | | [http://be.mit.edu/directory/angela-belcher AB]<br> | + | | [http://be.mit.edu/directory/angela-belcher AB]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Purify phage-based active (cathode) material (Day2) | Purify phage-based active (cathode) material]]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Homework#Due_M3D2| Homework due]] |
|-- | |-- | ||
| 3 | | 3 | ||
| Line 195: | Line 195: | ||
| T/W Apr/May 30/1 | | T/W Apr/May 30/1 | ||
| [http://be.mit.edu/directory/angela-belcher AB]<br> | | [http://be.mit.edu/directory/angela-belcher AB]<br> | ||
| − | | [[20.109( | + | | [[20.109(S20):Construct cathode with phage-based active material (Day3)| Construct cathode with phage-based active material]]<br> |
| − | | <font color= # | + | | <font color= #00BFFF>'''Laboratory quiz'''</font color> <br> [[20.109(S20):Homework#Due_M3D3| Homework due]] |
|-- | |-- | ||
| 3 | | 3 | ||
| 4 | | 4 | ||
| R/F May 2/3 | | R/F May 2/3 | ||
| − | | [http://be.mit.edu/directory/angela-belcher AB]<br> | + | | [http://be.mit.edu/directory/angela-belcher AB]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Visualize phage nanowires using TEM (Day4)| Visualize phage nanowires using transmission electron microscopy]]<br> |
| − | | [[20.109( | + | | [[20.109(S20):Homework#Due_M3D4| Homework due]] |
|-- | |-- | ||
| 3 | | 3 | ||
| Line 209: | Line 209: | ||
| T/W May 7/8 | | T/W May 7/8 | ||
| [http://be.mit.edu/directory/angela-belcher AB] | | [http://be.mit.edu/directory/angela-belcher AB] | ||
| − | | [[20.109( | + | | [[20.109(S20):Assemble and test batteries (Day5) | Assemble and test batteries ]]<br> |
| − | | <font color= # | + | | <font color= #00BFFF>'''Laboratory quiz'''</font color> <br> [[20.109(S20):Homework#Due_M3D5| Homework due]] |
|-- | |-- | ||
| | | | ||
| Line 216: | Line 216: | ||
| R/F May 9/10 | | R/F May 9/10 | ||
| | | | ||
| − | | [[20.109( | + | | [[20.109(S20):Research proposal presentation | Research proposal presentations]] |
| − | | [https:/ | + | | [https:// Blog post due] Sat, May 11 at 10 pm |
|-- | |-- | ||
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| Line 224: | Line 224: | ||
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| <font color = #FACC2E>'''Feedback and celebratory lunch'''</font color> | | <font color = #FACC2E>'''Feedback and celebratory lunch'''</font color> | ||
| − | | [[20.109( | + | | [[20.109(S20): Mini-report | <font color=#FF0000>Mini-report due</font color>]] Mon, May 13 at 10 pm <br> |
|} | |} | ||
</div> | </div> | ||
Revision as of 19:12, 14 January 2020
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm (32-124)
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm (56-322)
