Difference between revisions of "20.109(S18): Spring 2018 schedule"

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<font color=#0000FF>'''SCHEDULE DETAILS'''</font color><br>
 
<font color=#0000FF>'''SCHEDULE DETAILS'''</font color><br>
<font color=#0000FF>'''Lecture:'''</font color><br> T/R 11-12 (16-220) <br>
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<font color=#0000FF>'''Lecture:'''</font color><br>T/R 11-12 (16-220)<br>
<font color=#0000FF>'''Laboratory sections:''' </font color><br> T/R 1-5 or W/F 1-5 (56-322)<br>
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<font color=#0000FF>'''Laboratory sections:'''</font color><br>T/R 1-5 or W/F 1-5 (56-322)<br>
  
 
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Revision as of 15:35, 4 January 2018

20.109(S18): Laboratory Fundamentals of Biological Engineering

Sp18 banner image v2.png

Spring 2018 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Assessing ligand binding        2. Measuring gene expression        3. Engineering biomaterials              


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.


SCHEDULE DETAILS
Lecture:
T/R 11-12 (16-220)
Laboratory sections:
T/R 1-5 or W/F 1-5 (56-322)

MODULE DAY DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
T/W Feb 6/7 NLL
Orientation
1 1 R/F Feb 8/9 AK
In silico cloning and confirmation digest
Orientation quiz
Homework due
1 2 T/W Feb 13/14 AK
Complete small microarry analysis and induce protein expression
Homework due
1 3 R/F Feb 15/16 AK
Purify protein
Homework due
T/W Feb 20/21 President's day holiday
1 4 R/F Feb 22/23 AK
Assess protein purity and concentration
Laboratory quiz
Homework due
1 5 T/W Feb 27/28 AK
SECONDARY ASSAY D1
Homework due
1 6 R/F Mar 1/2 AK
SECONDARY ASSAY D2
Homework due
1 7 T/W Mar 6/7 AK
Complete data analysis
Laboratory quiz
Homework due
2 1 R/F Mar 8/9 NLL
Practice tissue culture techniques and seed cells for RNA purification
Homework due
2 2 T/W Mar 13/14 NLL
Induce DNA damage and apply drug treatments for RNA purification
Homework due
Data Summary draft due DATE at 10 pm
Blog post due DATE at 10 pm
2 3 R/F Mar 15/16 EF
Purify RNA and practice RNA-seq data analysis methods
Homework due
Mini-presentation due DATE at 10 pm
2 4 T/W Mar 20/21 EF
Analyze RNA-seq data and prepare for quantitative PCR experiment
Laboratory quiz
Homework due
2 5 R/F Mar 22/23 EF
ANALYZE RNA SEQ DATA Homework due
T/W Mar 27/28 Spring vacation Data Summary revision due DATE at 10 pm
R/F Mar 29/30 Spring vacation
2 6 T/W Apr 3/4 Comm Lab
Journal club I presentations
2 7 R/F Apr 5/6 NLL
Journal club II presentations
Blog post due DATE at 10 pm
2 8 T/W Apr 10/11 EF
Induce DNA damage and apply drug treatments for cell viability and RNA_SEQ DATA ANALYSIS Homework due
2 9 R/F Apr 12/13 EF
Complete cell viability assay
Laboratory quiz
Homework due
T/W Apr 17/18 Patriots' day holiday
3 1 R/F Apr 19/20 AB
Grow phage-based active (cathode) material
Homework due
Research article due DATE at 10 pm
Blog post due DATE at 10 pm
3 2 T/W Apr 24/25 AB
Purify phage-based active (cathode) material
Homework due
3 3 R/F Apr 26/27 AB
Construct cathode with phage-based active material
Laboratory quiz
Homework due
3 T/W May 1/2 Lecture, but no laboratory
3 4 R/F May 3/4 AB Visualize phage nanowires using TEM
Homework due
3 5 T/W May 8/9 AB Assemble and test batteries
Laboratory quiz
Homework due
R/F May 10/11 Research proposal presentations Blog post due DATE at 10 pm
T May 15 Feedback and celebratory lunch Mini-report due DATE at 10 pm