Difference between revisions of "20.109(S18):Test protein activity using peptidyl-prolyl cis-trans isomerase assay (Day5)"

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Revision as of 00:40, 26 January 2018

20.109(S18): Laboratory Fundamentals of Biological Engineering

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Spring 2018 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Assessing ligand binding        2. Measuring gene expression        3. Engineering biomaterials              


Introduction

Protocols

Part 1: Prepare samples for PPIase assay

Reagents

Navigation links

Next day: Confirm ligand binding using differential scanning fluorimetry assay

Previous day: Evaluate protein purity and concentration
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