Difference between revisions of "20.109(S18):Confirm ligand binding using differential scanning fluorimetry assay (Day6)"
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===Part 2: Prepare samples for DSF assay=== | ===Part 2: Prepare samples for DSF assay=== | ||
| + | |||
| + | |||
| + | <center> | ||
| + | {| border="1" | ||
| + | ! Reagent (stock concentration) | ||
| + | ! Final concentration of stock reagent in reaction | ||
| + | ! Volume of stock reagent in reaction | ||
| + | |- | ||
| + | | FKBP12 (1 mg/mL) | ||
| + | | 1.2 μg/mL | ||
| + | | | ||
| + | |- | ||
| + | | DMSO (1%) | ||
| + | | 0.2% | ||
| + | | | ||
| + | |- | ||
| + | | rapamycin (50 μM) | ||
| + | | 10 μM | ||
| + | | | ||
| + | |- | ||
| + | | ligand (200 μM) | ||
| + | | 40 μM | ||
| + | | | ||
| + | |- | ||
| + | | dye (5X) | ||
| + | | 1X | ||
| + | | | ||
| + | |- | ||
| + | | PBS (1X) | ||
| + | | add for a total of 10 μL per reaction | ||
| + | | | ||
| + | |} | ||
| + | </center> | ||
==Reagents== | ==Reagents== | ||
Revision as of 22:02, 3 February 2018
Contents
Introduction
Protocols
Part 1: BE Communication Lab workshop
Our communication instructors, Dr. Sean Clarke and Dr. Prerna Bhargava, will join us today for a workshop on writing impactful abstracts and titles.
Part 2: Prepare samples for DSF assay
| Reagent (stock concentration) | Final concentration of stock reagent in reaction | Volume of stock reagent in reaction |
|---|---|---|
| FKBP12 (1 mg/mL) | 1.2 μg/mL | |
| DMSO (1%) | 0.2% | |
| rapamycin (50 μM) | 10 μM | |
| ligand (200 μM) | 40 μM | |
| dye (5X) | 1X | |
| PBS (1X) | add for a total of 10 μL per reaction |
Reagents
Next day: Complete data analysis
