20.109(S16):In situ cloning (Day1)
From Course Wiki
Revision as of 15:04, 5 January 2016 by Noreen Lyell (Talk | contribs)
Introduction
[[Image:Sp16 M1D1 cloning schematic.png|thumb|center|400px|Schematic of pRSET-IPC cloning. The EGFP insert is PCR amplified then ligated into the pRSET vector using a compatible restriction sequence, EcoRI.
Next day: Design mutation primers
Previous day: Orientation