20.109(S13): Primer design summary

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20.109(S13): Laboratory Fundamentals of Biological Engineering

S13 109-OWW-frontpage.jpg

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Overview

You will write a brief memo about your study of diagnostic primers for identifying microsporidia.

The target audience is an advisor for whom you are preparing a relatively informal (but accurate!) report.

Logistics

Due date?!

Content Guidelines

Two primary sections -- one on design and one on results -- will make up the memo.

Design

Here you should include the following:

  • A short (1-2 paragraph) introduction motivating your design challenge and explaining your design choices.
  • A table comparing the properties of the V1-PMP2 primer set and your primer set.

The text related to your specific design choices should explicitly refer to the table.

Results

Here you should include the following:

  • A short (1-2 paragraph) description of your findings.
  • A figure depicting your raw PCR results (as seen on a gel).
  • Optionally, a figure or table depicting the PCR results in some processed form.
The figure should include a complete caption as for a publication.