20.109(S11): TA notes for module 2

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20.109(S11): Laboratory Fundamentals of Biological Engineering

20.109(S11) frontpg.JPG

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General notes

Key preparation:


Scheme:

Day-by-day

Day 1

Materials required:

Day of Lab (X/Y):

After Lab

How it went:

Day 2

Etc.

Special materials

Cell strains

  • NB399 = JW3367c (EnvZ-, lacZ-, Kan^R removed)
  • NB435/AB? = pEDL3, pCph8, pPL-PCB (full ED system)
  • NB? = light to lacZ only
  • AB? = pED-IPTG-110
  • AB? = pED-IPTG-112
  • AB? = pJT104 (full ED system less AHL)
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