Difference between revisions of "20.109(S11):Start-up biomaterials engineering (Day1)"
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==Protocols== | ==Protocols== | ||
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Half the class today will start in the cell culture facility and half will start with experimental design. Midway through class, you'll switch places. In your notebooks, you should only write up Part 2 of the protocol. For Part 1, the only thing you need to write down is your cell count data, for use in a later FNT calculation. The Part 1 protocol will be posted on each tissue culture hood for your reference. | Half the class today will start in the cell culture facility and half will start with experimental design. Midway through class, you'll switch places. In your notebooks, you should only write up Part 2 of the protocol. For Part 1, the only thing you need to write down is your cell count data, for use in a later FNT calculation. The Part 1 protocol will be posted on each tissue culture hood for your reference. | ||
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#While you are waiting, you can add 1 ml of sterile 0.1% gelatin to the two leftmost wells of a six-well dish (one dish per pair) and also label the dish. | #While you are waiting, you can add 1 ml of sterile 0.1% gelatin to the two leftmost wells of a six-well dish (one dish per pair) and also label the dish. | ||
#*The gelatin will be removed before you seed your MES cells but it is important to pre-treat the dish this way. MES must grow on either a "feeder layer" of fibroblasts, or on a gelatin-coated surface. '''The pre-treatment must be done for at least 10 minutes.''' | #*The gelatin will be removed before you seed your MES cells but it is important to pre-treat the dish this way. MES must grow on either a "feeder layer" of fibroblasts, or on a gelatin-coated surface. '''The pre-treatment must be done for at least 10 minutes.''' | ||
− | #*Label the plate lid with your group colour, | + | #*Label the plate lid with your group colour, today s date, and the cell line (called [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=SCRC-1010&Template=cellBiology |