20.109(F20):M1D5
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Part 2: Learn about the CometChip
As discussed in the prelab lecture, you will use two methods to assess DNA damage: the γH2AX assay and the CometChip. In the next laboratory session you will construct a CometChip; however, it is best to familiarize yourself with the procedure and assay before then.
Read the Abstract and Introduction in the following publication:
CometChip: A high-throughput 96-well platform for measuring DNA damage in microarrayed human cells. Journal of Visualized Experiments. (2014) 92: 1-11.
Answer the following questions (be sure to transfer the information to your laboratory notebook!):
- Why is it important to study DNA damage?
- You can consider the information provided in lecture / prelab to answer this question.
- How does the CometChip estimate the level of DNA damage within cells?
- List two issues / problems with the comet assay?
- List two improvements provided by the CometChip assay?
Next, watch the following video to help you prepare for the next laboratory session: linked here.
