20.109(F18): Fall 2018 schedule
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm (4-149)
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm (56-322)
| MODULE | DAY | DATE | LECTURER | LABORATORY EXPERIMENTS | ASSIGNMENTS | |
| R/F Sept 6/7 | NLL |
Orientation |
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| 1 | 1 | T/W Sept 11/12 | BE | Laboratory orientation quiz Homework due | ||
| 1 | 2 | R/F Sept 13/14 | BE | Homework due | ||
| 1 | 3 | T/W Sept 18/19 | BE | Homework due | ||
| 1 | R/F Sept 20/21 | Lecture, but no laboratory Career fair student holiday |
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| 1 | 4 | T/W Sept 25/26 | BE | Homework due | ||
| 1 | 5 | R/F Sept 27/28 | BE | Homework due | ||
| 1 | 6 | T/W Oct 2/3 | BE | Homework due | ||
| 1 | 7 | R/F Oct 4/5 | BE | Laboratory quiz Homework due | ||
| T/W Oct 10/11 | Columbus day holiday | Data Summary draft due at 10 pm | ||||
| 2 | 1 | R/F Oct 11/12 | NLL |
Complete in silico cloning |
Homework due Mini-presentation due at 10 pm | |
| 2 | 2 | T/W Oct 16/17 | NLL |
Design gRNA for CRISPRi |
Homework due | |
| 2 | 3 | R/F Oct 18/19 | NLL |
Generate gRNA plasmid |
Homework due Data Summary revision due at 10 pm | |
| 2 | 4 | T/W Oct 23/24 | NLL |
Journal club I | [ Blog post due] at 10 pm | |
| 2 | 5 | R/F Oct 25/26 | NLL |
Confirm gRNA sequence |
Laboratory quiz Homework due | |
| 2 | 6 | T/W Oct 30/31 | NLL |
Journal club II | ||
| 2 | 7 | R/F Nov 1/2 | NLL |
Induce CRISPRi system |
Homework due | |
| 2 | 8 | T/W Nov 6/7 | NLL |
Measure fermentation products |
Laboratory quiz Homework due | |
| 3 | 1 | T/W Nov 8/9 | AB | Prepare phage materials |
Homework due | |
| R/F Nov 12/13 | Veterans' day holiday | |||||
| 3 | 2 | R/F Nov 15/16 | AB | Purify active material for cathode construction |
Homework due | |
| T/W Nov 20/21 | AB |
Lecture, but no laboratory | Research article due at 10 pm [ Blog post due] at 10 pm | |||
| R/F Nov 22/23 | Thanksgiving holiday | |||||
| 3 | 3 | T/W Nov 27/28 | AB | Construct cathode with active material |
Laboratory quiz Homework due | |
| 3 | 4 | R/F Nov/Dec 29/30 | AB |
Visualize active material using TEM |
Homework due | |
| 3 | 5 | T/W Dec 4/5 | AB |
Assemble and test batteries |
Laboratory quiz Homework due | |
| 3 | 6 | R/F Dec 6/7 | Research proposal presentations | Research proposal presentation slides due Thu/Fri, Dec 6/7 at 1 pm [ Blog post due] at 10 pm | ||
| T Dec 11 | Feedback and celebratory lunch | Biomaterials engineering mini-report due at 10 pm [ Blog post due] at 10pm | ||||
