20.109(F17): Notes for M1

M1D1

Per group, aliquot in Tissue Culture, sterile

• 14ml of DMEM media
• 3ml of PBS
• 1ml of trypsin
• set aside T75 flask

Per group

• 50 mL 1x PBS in bottle
• cylinder near weight station for PBS
• 100ml glass bottles near weigh station
• pre-cut gel bond at front bench
• Secure line II pen

M1D2

Per group, aliquot

• in main lab
  • 1x PBS glass bottle
  • 2 eppendorf tubes of LMP agarose (in eppendorf water bath)
  • print a large scheme of the plate so they can keep track of how to load chips

• and separately, in the TC hood,
  • 5 mL 1x PBS
  • 10 mL MEF media

• 4 binder clips
• 1 glass slide
• 1 bottomless 96-well plate
• 1 razor blade
• Pasteur pipets
• sharp jar
• pipet aid

• team sticker to identify glass slides
• printout of today's protocol
• large scheme of the plate to hang in TC so they can keep track of how to load chips