Difference between revisions of "20.109(F17): Notes for M1"
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(→M1D1) |
(→M1D2) |
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===M1D1=== | ===M1D1=== | ||
Per group, aliquot in Tissue Culture, sterile | Per group, aliquot in Tissue Culture, sterile | ||
| − | *14ml of | + | *14ml of MEF media (DMEM, 10% FBS, 1X Pen/Strep) |
*3ml of PBS | *3ml of PBS | ||
*1ml of trypsin | *1ml of trypsin | ||
*set aside T75 flask | *set aside T75 flask | ||
| + | *printout of today's protocol | ||
Per group | Per group | ||
| Line 15: | Line 16: | ||
*pre-cut gel bond at front bench | *pre-cut gel bond at front bench | ||
*Secure line II pen | *Secure line II pen | ||
| + | *scraped lid for comet chip stamp | ||
===M1D2=== | ===M1D2=== | ||
| − | Per group, | + | Per group, in Tissue Culture, sterile |
| − | + | *10 mL MEF media | |
| − | * | + | *1ml trypsin |
| − | * | + | *5ml of PBS |
| − | * | + | *printout of today's protocol |
| − | + | ||
| − | + | ||
| − | + | ||
| − | * | + | |
| + | Per group, in main lab | ||
| + | *1x PBS in glass bottle | ||
| + | *2 eppendorf tubes of LMP agarose (in eppendorf water bath) | ||
*4 binder clips | *4 binder clips | ||
*1 glass slide | *1 glass slide | ||
| Line 34: | Line 35: | ||
*sharp jar | *sharp jar | ||
*pipet aid | *pipet aid | ||
| − | + | *40ml alkaline lysis solution | |
| − | * | + | *40ml Neutralization buffer |
| − | * | + | *print a large scheme of the plate so they can keep track of how to load chips |
| − | *large scheme of the plate | + | |
Latest revision as of 18:12, 11 September 2017
M1D1
Per group, aliquot in Tissue Culture, sterile
- 14ml of MEF media (DMEM, 10% FBS, 1X Pen/Strep)
- 3ml of PBS
- 1ml of trypsin
- set aside T75 flask
- printout of today's protocol
Per group
- 50 mL 1x PBS in bottle
- cylinder near weight station for PBS
- 100ml glass bottles near weigh station
- pre-cut gel bond at front bench
- Secure line II pen
- scraped lid for comet chip stamp
M1D2
Per group, in Tissue Culture, sterile
- 10 mL MEF media
- 1ml trypsin
- 5ml of PBS
- printout of today's protocol
Per group, in main lab
- 1x PBS in glass bottle
- 2 eppendorf tubes of LMP agarose (in eppendorf water bath)
- 4 binder clips
- 1 glass slide
- 1 bottomless 96-well plate
- 1 razor blade
- Pasteur pipets
- sharp jar
- pipet aid
- 40ml alkaline lysis solution
- 40ml Neutralization buffer
- print a large scheme of the plate so they can keep track of how to load chips
