Difference between revisions of "20.109(F17): Notes for M1"
From Course Wiki
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===M1D1=== | ===M1D1=== | ||
| − | Per group, aliquot | + | Per group, aliquot in Tissue Culture, sterile |
| − | *50 mL 1x PBS | + | *14ml of DMEM media |
| − | * | + | *3ml of PBS |
| + | *1ml of trypsin | ||
| + | *set aside T75 flask | ||
| + | |||
| + | Per group | ||
| + | *50 mL 1x PBS in bottle | ||
| + | *cylinder near weight station for PBS | ||
| + | *100ml glass bottles near weigh station | ||
| + | *pre-cut gel bond at front bench | ||
| + | |||
===M1D2=== | ===M1D2=== | ||
Per group, aliquot | Per group, aliquot | ||
Revision as of 17:40, 11 September 2017
M1D1
Per group, aliquot in Tissue Culture, sterile
- 14ml of DMEM media
- 3ml of PBS
- 1ml of trypsin
- set aside T75 flask
Per group
- 50 mL 1x PBS in bottle
- cylinder near weight station for PBS
- 100ml glass bottles near weigh station
- pre-cut gel bond at front bench
M1D2
Per group, aliquot
- in main lab
- 10 mL 1x PBS
- 2 eppendorf tubes of 0.3% LMP agarose (in eppendorf water bath)
- large scheme of the plate so they can keep track of how to load chips
- and separately, in the TC hood,
- 10 mL 1x PBS
- 25 mL TK6 media
- 2 eppendorf tubes of 0.3% LMP agarose (in water bath at 43 °C)
- 2x 15 mL of TK6 cells at 500,000 cells/mL
Set up hoods in TC room with, per group:
- 4 binder clips
- 1 glass slide
- 1 bottomless 96-well plate
- 1 razor blade
- Pasteur pipets
- sharp jar
- pipet aid
- pen
- team sticker to identify glass slides
- printout of today's protocol
- large scheme of the plate to hang in TC so they can keep track of how to load chips
