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| − | In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in ''E. coli''. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of ''E. coli'' such that either ethanol or | + | In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in ''E. coli''. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of ''E. coli'' such that either ethanol or acetate production is increased. |
Latest revision as of 22:28, 9 October 2017
Contents
Module 2
Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain and Josephine Bagnall
TA: Eric Lehnhardt
Lab manager: Hsinhwa Lee
Overview
In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in E. coli. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of E. coli such that either ethanol or acetate production is increased.
Lab links: day by day
M2D1: Complete in silico cloning
M2D2: Design gRNA for CRISPRi
M2D3: Generate gRNA plasmid
M2D4: Journal club I
M2D5: Confirm gRNA sequence
M2D6: Journal club II
M2D7: Induce CRISPRi system
M2D8: Measure fermentation products
Assignments
Research article
Journal club presentation
References
CRISPR interference (CRISPRi) for sequence-specific control of gene expression, (2013) by Larson et al.
