Difference between revisions of "20.109(F17):Module 2"
Noreen Lyell (Talk | contribs) (→Module 2) |
Noreen Lyell (Talk | contribs) (→Lab links: day by day) |
||
Line 18: | Line 18: | ||
==Lab links: day by day== | ==Lab links: day by day== | ||
− | [[20.109(F17):Complete in silico cloning (Day1)| | + | M2D1: [[20.109(F17):Complete in silico cloning (Day1)| Complete ''in silico'' cloning]]<br> |
− | [[20.109(F17):Design gRNA for CRISPRi (Day2)| | + | M2D2: [[20.109(F17):Design gRNA for CRISPRi (Day2)| Design gRNA for CRISPRi]]<br> |
− | [[20.109(F17):Generate gRNA plasmid (Day3)| | + | M2D3: [[20.109(F17):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br> |
− | [[20.109(F17):Journal club I (Day4)| | + | M2D4: [[20.109(F17):Journal club I (Day4)| Journal club I]]<br> |
− | [[20.109(F17):Confirm gRNA sequence (Day5)| | + | M2D5: [[20.109(F17):Confirm gRNA sequence (Day5)| Confirm gRNA sequence]]<br> |
− | [[20.109(F17):Journal club II (Day 6) | | + | M2D6: [[20.109(F17):Journal club II (Day 6) | Journal club II]]<br> |
− | [[20.109(F17):Induce CRISPRi system (Day7)| | + | M2D7: [[20.109(F17):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br> |
− | [[20.109(F17)::Measure fermentation products (Day8)| | + | M2D8: [[20.109(F17)::Measure fermentation products (Day8)| Measure fermentation products]] |
==Assignments== | ==Assignments== |
Revision as of 13:03, 6 September 2017
Contents
Module 2
Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain and Josephine Bagnall
TA: Eric Lehnhardt
Lab manager: Hsinhwa Lee
Overview
In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in E. coli. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of E. coli such that either ethanol or lactate production is increased.
Lab links: day by day
M2D1: Complete in silico cloning
M2D2: Design gRNA for CRISPRi
M2D3: Generate gRNA plasmid
M2D4: Journal club I
M2D5: Confirm gRNA sequence
M2D6: Journal club II
M2D7: Induce CRISPRi system
M2D8: Measure fermentation products
Assignments
Research article
Journal club presentation
References
CRISPR interference (CRISPRi) for sequence-specific control of gene expression, (2013) by Larson et al.