20.109(F17):Evaluate cell loading results (Day3)
Contents
Introduction
Protocols
Part 1: BE Communication Lab workshop
Our communication instructors, Dr. Sean Clarke and Dr. Prerna Bhargava, will join us today for a workshop on designing effective figures and captions.
Part 2: Image cell loading experiment
In the previous laboratory session you used the light microscope in the teaching laboratory to image your CometChip and determine the number of wells that were loaded and how many cells were in each of the wells. Today you will use a fluorescent microscope in the Engelward Laboratory to confirm that enough cells were loaded to allow for the visualization of the DNA in the microwells. This will also allow you to practice the image processing steps that you will use to examine your next CometChip experiment.
The teaching faculty will take one team at a time to the microscope in the Engelward Laboratory and demonstrate how the CometChip wells are imaged. While you are waiting for your turn, complete the image processing steps below with the images collected by the teaching faculty and posted to the Discussion tab of the Overview page. Note: due to time constraints the teaching faculty imaged your entire CometChip and those images should be used for this exercise, the images you collect today are for demonstration purposes only.
Part 3: Prepare CometChips to test biochemical factors
Part 4: Determine cell loading number for subsequent experiments
Reagents list
Next day: Test role of biochemical factors in genomic stability
