Difference between revisions of "20.109(F16):Test comet chip loading variables (Day2)"
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===Part 1: Design experiment to optimize comet chip loading=== | ===Part 1: Design experiment to optimize comet chip loading=== | ||
| − | loading | + | You will design two experiments that test variables associated with loading cells into the microwells of your comet chip. In the first experiment you will consider the role of cell number. Specifically, how many cells should be added to each well to ensure the majority of the microwells are loaded? In addition, how many cells should be loaded into each microwell? The second experiment will interrogate the length of time needed for loading. |
===Part 2: Load comet chip=== | ===Part 2: Load comet chip=== | ||
Revision as of 17:45, 30 June 2016
Contents
Introduction
experimental design...
Protocols
Part 1: Design experiment to optimize comet chip loading
You will design two experiments that test variables associated with loading cells into the microwells of your comet chip. In the first experiment you will consider the role of cell number. Specifically, how many cells should be added to each well to ensure the majority of the microwells are loaded? In addition, how many cells should be loaded into each microwell? The second experiment will interrogate the length of time needed for loading.
Part 2: Load comet chip
Reagents
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