Difference between revisions of "20.109(F16):Module 2"
Noreen Lyell (Talk | contribs) (→Lab links: day by day) |
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[[20.109(F16):Journal club II (Day 6) | M2D6: Journal club II]]<br> | [[20.109(F16):Journal club II (Day 6) | M2D6: Journal club II]]<br> | ||
[[20.109(F16):Induce CRISPRi system (Day7)| M2D7: Induce CRISPRi system]]<br> | [[20.109(F16):Induce CRISPRi system (Day7)| M2D7: Induce CRISPRi system]]<br> | ||
| − | [[20.109(F16)::Measure fermentation products (Day8)| Measure fermentation products]] | + | [[20.109(F16)::Measure fermentation products (Day8)| M2D8: Measure fermentation products]] |
==Assignments== | ==Assignments== | ||
Latest revision as of 19:39, 3 November 2016
Contents
Module 2
Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain and Maxine Jonas
TA: Emily Clark
Lab manager: Hsinhwa Lee
Overview
In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in E. coli. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of E. coli such that either ethanol or lactate production is increased.
Lab links: day by day
M2D1: Complete in silico cloning
M2D2: Design gRNA for CRISPRi
M2D3: Generate gRNA plasmid
M2D4: Journal club I
M2D5: Confirm gRNA sequence
M2D6: Journal club II
M2D7: Induce CRISPRi system
M2D8: Measure fermentation products
Assignments
Research article
Journal club presentation
References
CRISPR interference (CRISPRi) for sequence-specific control of gene expression, (2013) by Larson et al.
