20.109(F15): TA's notes for module 3

Day 1

The week preceding the lab:

• Make LB:
  • 10 g tryptone
  • 5 g yeast extract
  • 10 g NaCl
  • 1 L deionized water
  • autoclave 30 minutes with stirbar.

On the instructors' bench:

• 50 mL conical tubes
• 25 mL pipets
• a couple of motorized pipet aids
• 2 mL eppendorf tubes
• TBS: 500 mL
• PEG/NaCl: 500 mL
• deionized water: 50 mL
• quartz cuvettes

On the students' bench:"

• bucket of ice
• 2 x 40 mL overnight culture of XL1-Blue infected with M13 phage
• 250 mL erlenmeyer flask

By the scale:

• (NH₄)₂Fe(SO₄)₂
• stirbars

In 4 °C fridge:

• orbital shaker (speed 120)