Difference between revisions of "20.109(F11): Mod 1 Day 5 Examine candidate clones & tissue culture"
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==Introduction== | ==Introduction== | ||
− | Yesterday a few milliliters of LB+AMP broth were inoculated with some candidate colonies and the tubes were grown overnight at 37°C. Ampicillin was included in the broth to ensure that the cells would maintain the plasmid DNA. Today the cells will have grown to high density and the plasmid DNA will have undergone many replications. All the copies of the plasmid DNA in your overnight culture should be identical ( | + | Yesterday a few milliliters of LB+AMP broth were inoculated with some candidate colonies and the tubes were grown overnight at 37°C. Ampicillin was included in the broth to ensure that the cells would maintain the plasmid DNA. Today the cells will have grown to high density and the plasmid DNA will have undergone many replications. All the copies of the plasmid DNA in your overnight culture should be identical ( |