Difference between revisions of "20.109(F11): Mod 1 Day 4 DNA ligations and bacterial transformations"
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(New page: {{Template:20.109(F11)}} <div style="padding: 10px; width: 640px; border: 5px solid #999999;"> =<center>DNA ligations and bacterial transformations</center>= ==Introduction== Today you wil...) |
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[[Image:Mod1 3 sche bact.jpg|thumb|right|400px|'''Bacterial transformation''']] | [[Image:Mod1 3 sche bact.jpg|thumb|right|400px|'''Bacterial transformation''']] | ||
− | If all goes well, your ligation reactions will generate your desired construct: the pCX-NXX backbone carrying the EGFP gene lacking the first 32 amino acids. You will transform this product into bacteria. During | + | If all goes well, your ligation reactions will generate your desired construct: the pCX-NXX backbone carrying the EGFP gene lacking the first 32 amino acids. You will transform this product into bacteria. During |