Difference between revisions of "20.109(F08): Protein engineering research article"
From Course Wiki
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Primer design in Materials and Methods<br> | Primer design in Materials and Methods<br> | ||
Figures to include: | Figures to include: | ||
| + | |||
*Agarose Gel showing transformed frag from PCR/Qkit | *Agarose Gel showing transformed frag from PCR/Qkit | ||
| + | *Transformation results | ||
*Western showing TAP | *Western showing TAP | ||
*Growth Phenotypes | *Growth Phenotypes | ||
Revision as of 00:25, 30 August 2008
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Primer design in Materials and Methods
Figures to include:
- Agarose Gel showing transformed frag from PCR/Qkit
- Transformation results
- Western showing TAP
- Growth Phenotypes
- Array data
- Figure N: Growth phenotypes. If you'd like an example of a spot test figure from a published article, consider modeling your figure and legend along the lines of Figure 2 from Monehan et al "Fission yeast SWI/SNF and RSC complexes show compositional and functional differences from budding yeast"Nature Structural & Molecular Biology (2008) 15, 873 - 880 here
- Figure N: Western blot for TAP tag. If you'd like an example of a figure that considers protein levels, consider modeling your figure and legend along the lines of figure 6 from Laprade et al. "Characterization of New Spt3 and TATA-Binding Protein Mutants of Saccharomyces cerevisiae: Spt3–TBP Allele-Specific Interactions and Bypass of Spt8" Genetics(2007)177: 2007-2017 here
