Difference between revisions of "20.109(F07): Phage by design, pt2"

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{{Template:20.109(F07)}}
 
{{Template:20.109(F07)}}
 
==Introduction==
 
==Introduction==
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[[Image:Macintosh HD-Users-nkuldell-Desktop-electrodeposition.png|400 px]]
 
==Protocols==
 
==Protocols==
 
===Part 1: M13.1 titers===
 
===Part 1: M13.1 titers===
 +
The strains you transformed last time with M13K07 and M13.1 were inoculated into LB+Kan and grown overnight. If the two forms of the phage are equally robust, then the phage titer in the liquid surrounding the cells should be approximately the same. Ditto if the wild type strain, MG1655, and the MDS strain are equally capable of phage production. You will compare the PFU/ul of each culture to determine this. The protocol is identical to the one from [[20.109(F07): Agarose gel electrophoresis#Protocols| module 1]]. Since you don't know if the phage production will be improved or diminished by any of the variations, you will titer a wider-than-usual range of concentrations.
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<center>
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{| border="1"
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|
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|phage = M13K07, strain = MG1655 
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|phage = M13K07, strain = MDS
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|phage = M13.1, strain = MG1655
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|phage = M13.1, strain = MDS
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|-
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| 10-6
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|
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|
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|
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|
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|-
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| 10-8
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|
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|
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|
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|
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|-
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| 10-10
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|
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|
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|
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|
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|}
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</center>
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You should also include a no phage control plate for confidence that the number of plaques you see next time arise as a result of your experiment.
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 +
 
===Part 2: Phage deposition onto patterned slide===
 
===Part 2: Phage deposition onto patterned slide===
−
[[Image:Macintosh HD-Users-nkuldell-Desktop-electrodeposition.png|400 px]]
+
 
 
DONE!
 
DONE!
 
==For next time==
 
==For next time==

Revision as of 18:04, 2 November 2007


20.109(F07): Laboratory Fundamentals of Biological Engineering

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Introduction

400 px

Protocols

Part 1: M13.1 titers

The strains you transformed last time with M13K07 and M13.1 were inoculated into LB+Kan and grown overnight. If the two forms of the phage are equally robust, then the phage titer in the liquid surrounding the cells should be approximately the same. Ditto if the wild type strain, MG1655, and the MDS strain are equally capable of phage production. You will compare the PFU/ul of each culture to determine this. The protocol is identical to the one from module 1. Since you don't know if the phage production will be improved or diminished by any of the variations, you will titer a wider-than-usual range of concentrations.

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6
10-8
10-10

You should also include a no phage control plate for confidence that the number of plaques you see next time arise as a result of your experiment.


Part 2: Phage deposition onto patterned slide

DONE!

For next time

  1. water
  2. how to more densely pack nanowires

Reagents list

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