20.109(F07): Genome engineering assessment

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20.109(F07): Laboratory Fundamentals of Biological Engineering

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Portfolio components

Part 1:Rebuttal to editorial.

This will be written as a homework assignment, exchanged with your lab partner for peer review, then submitted to the teaching faculty as part of your portfolio

Part 2: M13.1 redesign description and parts

Part 3: Data summary for p3-modifications you performed in lab

Including but not limited to:

  • Oligonucleotide design, sequence consequences for phage when inserted and sequence data
  • Ligation results (table)
  • Agarose gel examining candidate clones (figure)
  • Western results (figure)
  • Plaque assay (figure)
  • Short paragraph for each table and figure describing and interpreting what's shown
  • One or two sentence summary of your experimental results
  • One or two sentence proposal for what you'd do next if we had one more month to spend on this project

Part 4: Registry of Standard Biological Parts

  • Existing strengths/usefulness
  • Measures for success of registry
  • Suggestions for improvement