20.109(S18): Prep notes for M1
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Revision as of 21:21, 31 January 2018 by Noreen Lyell (Talk | contribs)
M1D1
- restriction enzymes and buffers
M1D2
prior to laboratory:
- prepare 1% agarose gels, 1 per 2 teams
- subculture BL21(DE3) pRSETb_FKBP12 1:10 into 50 mL LB containing 100μg / mL amp and 34 μg / mL cam, 1 per team
on front bench (per team):
- X μL 6x loading dye
- Y μL 1 M IPTG
M2D2
on front bench (per team):
- lysis buffer reagents
- 250 μL slurry
