Spring 11:QRT-PCR
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Revision as of 23:28, 20 March 2011 by Kristin Kuhn (Talk | contribs)
Looking for weekly reports?
To-Do
- research what to order for heated lid
Weekly goals
- 3/25: Optics finished. Code started.
- 4/1: Heated lid finished.
- 4/8: Updated heat transfer function. Successful PCR.
- 4/15: QRT-PCR code finished.
- 4/22: Setup is ready for QRT-PCR. (i.e., the mechanical setup works)
- 4/29: Successful QRT-PCR from the side.
- 5/6: QRT-PCR from the top. Now which one was better?
Progress Notes
3/20/11
Based on model system (blue LED + ND filter), I don't think gain is high enough. Increased gain of transimpedance amp from 2.5e4 to 2.5e5.
3/18/11
Optical setup appears to be working.
3/16/11
Added dichroic mirror.
Question: how can I verify the optical setup? it seems to respond to driving signal, not fluorescence.
TODO: put away 25mm lens
