Talk:20.109(F16):Module 2
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Contents
Module 2 Data
M2D2: gRNA design
Fermentation product and gene targeted:
T/R: lactate
| Team | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) | Locus targeted (e.g. beginning of gene, putative promoter, -35 region) |
| yellow | pflB | TGTCGAAGTACGCAGTAAAT | |
| green | pflB | ATAAAAAATCCACTTAAGAAGGTA | |
| blue | pflB | TTCATTAAGCTCGGACATGTAACA | |
| pink | pflB | AAATAAAAAATCCACTTAAGAAGGT | |
| purple | pflB | AAATCCACTTAAGAAGGTAGGTGT |
W/F: ethanol
| Team | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) | Locus targeted (e.g. beginning of gene, putative promoter, -35 region) |
| red | pta-ack | GCC ACG TAT CAA TTA TAG GTA C | |
| green | ldhA | GTAGCTTAAATGTGATTCAACATC | |
| blue | fdrA | CAAGATCGGCTTGAAAGGTTTGCAC | |
| purple | ldhA | TCGTACTGTTTTGTGCTATAAA |
