20.109(S14): Choose system conditions and paper discussion (Day3)

Introduction

brief context

topic 1: restriction enzymes

topic 2: design considerations in a repair report assay

Protocols

Part 1: Paper discussion

Coming soon!

Technical Background

The main technical topic that may be unfamiliar to (some of) you is the use of short interfering RNA (siRNA). RNA interference, or RNAi, is a post-transcriptional silencing mechanism. You may have heard of gene "knock-outs" before, and in fact xrs6 are essentially Ku80 knock-outs: the DNA sequence is deleted or modified so as to be non-functional. In contrast, RNAi is usually not as potent, and hence is called gene "knock-down." Here the DNA sequence is normal, and mRNA is transcribed – but it is bound up or destroyed before it can be translated. You can read more about the intricacies of RNAi at Scitable.

Discussion Topics

Writing

• How does this abstract style differ from the one you have previously encountered – and also explicitly been taught – in 20.109? How is it the same? What are the pros and cons of each format? As a whole, did the abstract make you want to read the paper?

Content

Part 2: Reverse engineering pMax-BFP-MCS

Coming later!

Part 3: Choose system conditions

On today's Talk page...

For next time

digest planning and calculations in advance

Reagent list

Just your brains!

Navigation Links

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