20.109(F07): Genome engineering assessment
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Contents
Portfolio components
Part 1:Rebuttal to editorial.
This will be written as a homework assignment, exchanged with your lab partner for peer review, then submitted to the teaching faculty as part of your portfolio
Part 2: M13.1 redesign description and parts
Part 3: Data summary for p3-modifications you performed in lab
Including but not limited to:
- Oligonucleotide design, sequence consequences for phage when inserted and sequence data
- Ligation results (table)
- Agarose gel examining candidate clones (figure)
- Western results (figure)
- Plaque assay (figure)
- Short paragraph for each table and figure describing and interpreting what's shown
- One or two sentence summary of your experimental results
- One or two sentence proposal for what you'd do next if we had one more month to spend on this project
Part 4: Registry of Standard Biological Parts
- Existing strengths/usefulness
- Measures for success of registry
- Suggestions for improvement