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• 20.109(S07): Start-up signal measurement
  ...e chosen a PDB ID (singular or plural), go back to the center panel at the bottom of the Protein Explorer front door and type this code into the box associat
  21 KB (3,477 words) - 15:31, 15 June 2015
• 20.109(S07): Measuring calcium in vitro
  7. Wipe the top and bottom sensor with a water soaked Chemwipe.<br> 8. Dry the top and bottom sensor with a dry Chemwipe.<br>
  20 KB (3,237 words) - 15:45, 15 June 2015
• 20.109(S07): Calcium signaling in vivo
  ...e, top plot), and the fractional saturation of the calcium indicator (red, bottom plot). If you were doing actual fluorescence experiments, the signal chang
  14 KB (2,275 words) - 15:31, 15 June 2015
• 20.109(F07): siRNA design
  ...equence from the MSWord document you started into the box that is near the bottom of the webpage. Delete any numbers once you’ve pasted the sequence. Choos ...u submit your query, several target sequences will appear as a list at the bottom of the webpage. The candidate sequences are listed according to where they
  21 KB (3,532 words) - 15:54, 15 June 2015
• 20.109(F07):Lab tour
  ...our P20, measure 10, 15 and 20 l of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 l of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  22 KB (3,670 words) - 15:54, 15 June 2015
• 20.109(F07): DNA ligation and bacterial transformation
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  16 KB (2,748 words) - 15:54, 15 June 2015
• 20.109 (F07): Phage by design
  ...s being used for both bottom up and top down re-engineering of cells. In a bottom up approach, the genetic components for a “minimal cell” would be speci
  17 KB (2,797 words) - 15:54, 15 June 2015
• 20.109(F08):Lab tour
  ...our P20, measure 10, 15 and 20 l of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 l of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  26 KB (4,345 words) - 19:05, 28 July 2015
• 20.109(F08): Phage by design
  ...s being used for both bottom up and top down re-engineering of cells. In a bottom up approach, the genetic components for a “minimal cell” would be speci
  17 KB (2,797 words) - 19:05, 28 July 2015
• 20.109(F08): Start-up expression engineering
  ... sequence and whether you have the "top" strand as you did before, or the "bottom" strand as you will need for this reverse primer. [[Image:Macintosh HD-User
  29 KB (4,451 words) - 19:05, 28 July 2015
• 20.109(F08):DNA engineering/Clean and cut DNA (Day 2)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for EcoRI (see also fi ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is your PCR product, ready to be digested.
  10 KB (1,692 words) - 19:05, 28 July 2015
• 20.109(F08): Probe Western
  ...09" to login (Wed/Fri section: use "astachow" and "be109" instead). At the bottom of the left panel should be a link to download your sequencing results. Sel
  14 KB (2,285 words) - 19:05, 28 July 2015
• 20.109(F08):DNA engineering/DNA engineering by PCR (Day 1)
  ... manually adjust the margins of your document so they are 0.6 inches (top, bottom, left and right) and you should change the text to 10 point Courier font. C ...ll be assembling the components of the “D32N-fwd primer 5’- ” at the bottom of your MSWord document. There are several things to consider as you design
  23 KB (3,892 words) - 19:30, 28 July 2015
• 20.109(F08):DNA engineering/Agarose gel electrophoresis (Day 3)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  10 KB (1,806 words) - 19:05, 28 July 2015
• 20.109(F08):DNA engineering/Examine candidate clones (Day 5)
  ...or, but be careful not to disturb the pellet of plasmid DNA that is at the bottom of the tube. Remove as much of the supernatant as possible but you do not n ... to completely dissolve the pellets. The liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds. Store the
  10 KB (1,634 words) - 19:05, 28 July 2015
• 20.109(F08):DNA engineering/DNA ligation and bacterial transformation (Day 4)
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  10 KB (1,704 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 1 Day 2 Clean and cut DNA
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for EcoRI (see also fi ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is your PCR product, ready to be digested.
  10 KB (1,702 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 1 Day 1 DNA engineering using PCR
  ... manually adjust the margins of your document so they are 0.6 inches (top, bottom, left and right) and you should change the text to 10 point Courier font. C ...ll be assembling the components of the “D32N-fwd primer 5’- ” at the bottom of your MSWord document. There are several things to consider as you design
  22 KB (3,807 words) - 19:29, 28 July 2015
• 20.109(F08): Mod 1 Day 5 Examine candidate clones
  ...or, but be careful not to disturb the pellet of plasmid DNA that is at the bottom of the tube. Remove as much of the supernatant as possible but you do not n ... to completely dissolve the pellets. The liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds. Store the
  11 KB (1,770 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 1 Day 4 DNA ligations and bacterial transformations
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  10 KB (1,617 words) - 19:05, 28 July 2015