20.109(S22): Laboratory Fundamentals of Biological Engineering
Module 1: Drug discovery
SDS-PAGE gel images
[SDS-PAGE dropbox folder]
small molecule selections
T/R
Team
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small molecule #1 (enter compound ID)
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small molecule #2 (enter compound ID)
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aggregation assay results
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TR Red
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83079118
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69269200
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https://www.dropbox.com/s/7eut5ymxmiel9rp/TR_teal_red.xlsx?dl=0
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TR Orange
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95877382
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83023303
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TR Yellow
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69269200
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83079118
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TR Green
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69269200
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83079118
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TR Blue
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69269200
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83079118
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TR Teal
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69269200
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83079118
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TR Pink
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69269200
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83079118
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TR Purple
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69269200
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83079118
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TR Grey
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69269200
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83079118
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TR White
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69269200
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83079118
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W/F
Aggregation Results
[T/R Aggregation dropbox folder]
Module 2: Metabolic engineering
sgRNA_target sequences
T/R
Team
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Ethanol (E) or Acetate (A)
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Gene targeted by CRISPRi gRNA
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gRNA (DNA) sequence (without tag at 3' end)
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Locus targeted (eg. beginning of gene, putative promoter, -35 region)
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Target coding or non-coding strand
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Ethanol / Acetate Assay Results
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TR Red
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TR Orange
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TR Yellow
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TR Green
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TR Blue
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TR Teal
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TR Pink
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TR Purple
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TR Grey
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TR White
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W/F
Team
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Ethanol (E) or Acetate (A)
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Gene targeted by CRISPRi gRNA
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gRNA (DNA) sequence (without tag at 3' end)
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Locus targeted (eg. beginning of gene, putative promoter, -35 region)
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Target coding or non-coding strand
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Colorimetric Assay Results
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WF Red
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WF Orange
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WF Yellow
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WF Green
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WF Blue
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WF Teal
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WF Pink
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WF Purple
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WF Grey
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WF White
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