20.109(F07): ECD assembly
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Contents
Introduction
Protocol
Part 1: Phage titers for M13.1 and MDS
Record the number of plaques for each plate you prepared last time, and then use the most "countable" to calculate PFU/ml for the M13K07/M13.1 and WT/MDS pairs. To collect the class's data in one place, you should then copy two tables to the data collection page.
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | ||||
10-8 | ||||
10-10 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) |
Part 2: Li overlay
DONE!
For next time
- titer?, summary paragraph
- summary of ECD, variables and effects
- final presentation directions