Talk:20.109(F17):Design gRNA for CRISPRi (Day2)
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Fermentation product and gene targeted:
T/R
Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
red | Acetate | adhE | CTTAACGCACTCGTAGAGCGTGTAAA |
orange | Ethanol | pta-ack | CTATGGCTCCCTGACGTTTT |
yellow | Ethanol | frdA | GCTGTGGGATAAAAACAATCTGGAG |
green | E | LdhA | ttgtgctataaacggcgagtttcat |
blue | E | pta | ttcacgacaacgttcaataatcat |
pink | Acetate | adhE | TTACTAAAAAAGTTTAACATTATCA |
purple | Acetate | adhE | GATTTACTAAAAAAGTTTAACATTATC |
white | Acetate | adhE | CTGATAATGTTAAACTTTTT |
W/F
Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
red | |||
orange | Ethanol | ldhA | ATTCAACATCACTGGAGAAAGTCTT |
green | |||
blue | Ethanol | ackA | TTTTTAGCCACGTATCAATTAT |