Difference between revisions of "Assignment 2: fluorescence microscopy"
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# Is the Poisson distribution a good approximation of shot noise? | # Is the Poisson distribution a good approximation of shot noise? | ||
#* What percentage of the results fall with one, two, and three standard deviations? | #* What percentage of the results fall with one, two, and three standard deviations? | ||
− | #* Produce a log-log plot of standard deviation of the number of photons emitted as a function of the average number of photons emitted for probability of photon emission equal to the values: 10-6, 10-5, 10-4, 10-3, and 10-2. | + | #* Produce a log-log plot of standard deviation of the number of photons emitted as a function of the average number of photons emitted for probability of photon emission equal to the values: 10<sup>-6</sup>, 10<sup>-5</sup>, 10<sup>-4</sup>, 10<sup>-3</sup>, and 10<sup>-2</sup>. |
#* Hints: Use a nested loop. Use the poissrnd function instead of the line containing rand. Speed things up by getting rid of the plotting inside the loop and only run 100 simulations for each probability. | #* Hints: Use a nested loop. Use the poissrnd function instead of the line containing rand. Speed things up by getting rid of the plotting inside the loop and only run 100 simulations for each probability. | ||
#* What is the relationship between the number of photons detected and the noise (standard deviation)? | #* What is the relationship between the number of photons detected and the noise (standard deviation)? | ||
#* Turn in the code you used to generate the plots | #* Turn in the code you used to generate the plots | ||
− | # On one set of axes, plot the variance vs. mean for exposure times of 10-4, 10-3, 10-2, 10-1, 100 seconds. | + | # On one set of axes, plot the variance vs. mean for exposure times of 10<sup>-4</sup>, 10<sup>-3</sup>, 10<sup>-2</sup>, 10<sup>-1</sup>, and 100 seconds. |
# Using the camera measurements provided: | # Using the camera measurements provided: | ||
## Plot the raw data from the static scene measurements and the model best fit on one set of axes. | ## Plot the raw data from the static scene measurements and the model best fit on one set of axes. |
Revision as of 19:00, 31 January 2019
This assignment has 3 parts.
- Part 1: Noise in images
- Part 2: Fluorescence microscopy
- Part 3: Build an epi-illuminator for your microscope
Submit your work on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment2.pdf.
- Overview
- Part 1: Noise in images
- Part 2: Fluorescence microscopy
- Part 3: Build an epi-illuminator for your microscope
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