Difference between revisions of "Talk:20.109(F16):Module 2"
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MAXINE JONAS (Talk | contribs) (→Module 2 Data) |
MAXINE JONAS (Talk | contribs) (→M2D5/M2D7: sequencing data) |
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===M2D5/M2D7: sequencing data=== | ===M2D5/M2D7: sequencing data=== | ||
| − | ====W/F | + | ====T/R==== |
| + | |||
| + | {| border=1px | ||
| + | |'''Team''' | ||
| + | |'''pgRNA sequencing''' | ||
| + | |- | ||
| + | |yellow | ||
| + | |[[Media:TRyellow_sequencing.zip| files]] | ||
| + | |- | ||
| + | |green | ||
| + | |[[Media:TRgreen_sequencing.zip| files]] | ||
| + | |- | ||
| + | |blue | ||
| + | |[[Media:TRblue_sequencing.zip| files]] | ||
| + | |- | ||
| + | |pink | ||
| + | |[[Media:TRpink_sequencing.zip| files]] | ||
| + | |- | ||
| + | |purple | ||
| + | |[[Media:TRpurple_sequencing.zip| files]] | ||
| + | |} | ||
| + | |||
| + | ====W/F==== | ||
{| border=1px | {| border=1px | ||
Revision as of 14:27, 2 November 2016
Contents
Module 2 Data
M2D2: gRNA design
T/R: lactate
| Team | Gene targeted by CRISPRi gRNA |
gRNA sequence (without tag at 3' end) |
Locus targeted (e.g. beginning of gene, putative promoter, -35 region) |
| yellow | pflB | TGTCGAAGTACGCAGTAAAT | |
| green | pflB | ATAAAAAATCCACTTAAGAAGGTA | |
| blue | pflB | TTCATTAAGCTCGGACATGTAACA | |
| pink | pflB | AAATAAAAAATCCACTTAAGAAGGT | |
| purple | pflB | AAATCCACTTAAGAAGGTAGGTGT |
W/F: ethanol
| Team | Gene targeted by CRISPRi gRNA |
gRNA sequence (without tag at 3' end) |
Locus targeted (e.g. beginning of gene, putative promoter, -35 region) |
| red | pta-ack | GCC ACG TAT CAA TTA TAG GTA C | |
| green | ldhA | GTAGCTTAAATGTGATTCAACATC | |
| blue | fdrA | CAAGATCGGCTTGAAAGGTTTGCAC | |
| purple | ldhA | TCGTACTGTTTTGTGCTATAAA |
M2D5/M2D7: sequencing data
T/R
| Team | pgRNA sequencing |
| yellow | files |
| green | files |
| blue | files |
| pink | files |
| purple | files |
W/F
| Team | pgRNA sequencing |
| red | files |
| green | files |
| blue | files |
| purple | files |
