Difference between revisions of "Talk:20.109(S16):Purify protein (Day6)"
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===OD562 absorbance measurements for protein concentration calculations=== | ===OD562 absorbance measurements for protein concentration calculations=== | ||
− | + | During the 1 hour incubation of Part4A use excel to prepare a graph of absorbance versus BSA concentration using this sample data [[Media:SampleData_microBCA.xlsx| '''here'''.]] You will need to use the trendline equation to calculate the protein concentration of your example elution data. | |
====T/R red team==== | ====T/R red team==== | ||
{| border="1" | {| border="1" |
Revision as of 17:48, 22 February 2016
OD562 absorbance measurements for protein concentration calculations
During the 1 hour incubation of Part4A use excel to prepare a graph of absorbance versus BSA concentration using this sample data here. You will need to use the trendline equation to calculate the protein concentration of your example elution data.
T/R red team
name | OD562 |
standards | |
A | |
B | |
C | |
D | |
E | |
F | |
G | |
H | |
I | |
inverse pericam (IPC) | |
wild-type | |
mutant |