Difference between revisions of "20.109(S08): TA notes for module 1"
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==Daily Notes== | ==Daily Notes== | ||
− | ===[[20.109%28S08%29:DNA_engineering/ | + | ===[[20.109%28S08%29:DNA_engineering/DNA_engineering_by_PCR_%28Day_1%29 | Day 1]]=== |
'''Materials required:''' | '''Materials required:''' |
Revision as of 19:30, 27 December 2007
General notes
Plasmids used:
- pCX-EGFP:
- contains full-length EGFP gene
- Amp resistant
- f1 ori
- SV40 ori
- pCX-NNX:
- a mock version of pCX-EGFP, contains no EGFP gene
- Amp resistant
- f1 ori
- some altered restriction sites compared to pCX-EGFP, and SV40 ori deleted
Daily Notes
Day 1
Materials required:
- PCR Master Mix (2.5X), ~ 50 μL per group
- DI water, prep 100 μL aliquot per group
- 5 μL aliquots each of pCX-EGFP, D32N-fwd, D32N-rev
- 2 PCR tubes per group
Keep everything cold!
Day of Lab:
- Lab orientation quiz (not a TA quiz) will be taken today.
- Remember to freeze PCR products when they are ready.