Difference between revisions of "Using CRISPRi to increase ethanol yield in E. coli MG1655"
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Noreen Lyell (Talk | contribs) (→Related modules) |
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#*Provides background details on methods used for plasmid construction that are important in laboratory exercises focused on the steps after cloning | #*Provides background details on methods used for plasmid construction that are important in laboratory exercises focused on the steps after cloning | ||
#*Steps for optional at-the-bench confirmation digest laboratory exercise included | #*Steps for optional at-the-bench confirmation digest laboratory exercise included | ||
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#Optimizing sgRNA design to further increase ethanol yield (coming soon!) | #Optimizing sgRNA design to further increase ethanol yield (coming soon!) | ||
#*Students analyze datasets collected in previous semesters to design an optimized sgRNA sequence with laboratory exercises that can be completed in remote teaching format | #*Students analyze datasets collected in previous semesters to design an optimized sgRNA sequence with laboratory exercises that can be completed in remote teaching format |
Revision as of 14:35, 2 August 2020
Overview
Laboratory exercises
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Day 2:
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Day 5:
Related modules
- In silico cloning of pdCas9 construct
- Students perform steps used in classic cloning using free, online DNA manipulation interface with laboratory exercises that can be completed in remote teaching format
- Useful when timing doesn't allow for at-the-bench cloning laboratory exercise
- Provides background details on methods used for plasmid construction that are important in laboratory exercises focused on the steps after cloning
- Steps for optional at-the-bench confirmation digest laboratory exercise included
- Optimizing sgRNA design to further increase ethanol yield (coming soon!)
- Students analyze datasets collected in previous semesters to design an optimized sgRNA sequence with laboratory exercises that can be completed in remote teaching format